Multi-chambered receptacles
View Patent ↗A receptacle having a plurality of interconnected chambers arranged to permit multiple process steps or processes to be performed independently or simultaneously. The receptacles are manufactured to separate liquid from dried reagents and to maintain the stability of the dried reagents. An immiscible liquid, such as an oil, is included to control loading of process materials, facilitate mixing and reconstitution of dried reagents, limit evaporation, control heating of reaction materials, concentrate solid support materials to prevent clogging of fluid connections, provide minimum volumes for fluid transfers, and to prevent process materials from sticking to chamber surfaces. The receptacles can be adapted for use in systems having a processing instrument that includes an actuator system for selectively moving fluid substances between chambers and a detector. The actuator system can be arranged to concentrate an analyte present in a sample. The detector can be used to detect an optical signal emitted by the contents of the receptacle.
1. A multi-chambered receptacle for use in processing a sample, the receptacle comprising:
opposed members, wherein at least one of the opposed members comprises a flexible sheet;
a sample inlet port disposed on a top edge of the receptacle; and
a plurality of chambers having perimeter walls defined by permanent seals formed between the opposed members and portals interconnecting the chambers, wherein the plurality of chambers comprises:
a sample chamber disposed below the sample inlet port and in fluid communication with the sample inlet port;
a reagent chamber;
a process chamber directly connected with the sample chamber and directly connected with the reagent chamber; and
a waste chamber directly connected with only the process chamber, wherein the waste chamber includes an inlet region, a waste portal located at a top portion of the process chamber connecting the inlet region to the process chamber, wherein the inlet region is disposed above the waste portal, a collection region, and a dividing wall extending vertically upwardly from a part of the perimeter wall of the waste chamber between the inlet region and the collection region and terminating within the waste chamber, whereby a fluid material flowing within the waste chamber between the inlet region and the collection region must flow through an upper neck between the perimeter wall of the waste chamber and a terminal end of the dividing wall, wherein the upper neck is positioned above the inlet region and the collection region so that gravity prevents fluid material contained within the collection region from flowing upward through the upper neck and into the inlet region.
2. The receptacle of claim 1 , wherein one or more of the plurality of chambers comprises a solid support for immobilizing an analyte in the sample.
3. The receptacle of claim 2 , wherein the solid support comprises a magnetically-responsive particle or bead.
4. The receptacle of claim 1 , wherein at least one of the opposed members comprises a foil layer.
5. The receptacle of claim 1 , further comprising openable seals at the portals interconnecting two or more of the chambers, wherein the openable seals comprise peelable seals and wherein the peelable seals and the permanent seals are formed under different conditions, such that the peelable seals are weaker than the permanent seals.
6. The receptacle of claim 1 , further comprising openable seals at the portals interconnecting two or more of the chambers, wherein the openable seals are chevron seals.
7. The receptacle of claim 1 , wherein at least one of the chambers is a detection chamber directly connected to the process chamber and constructed and arranged to enable detection of a characteristic of a sample, wherein at least a portion of the detection chamber is constructed of optically transparent materials, thereby permitting the contents of the detection chamber to be interrogated by an optical sensor.
8. The receptacle of claim 7 , wherein the detection chamber contains one or more probes immobilized on an interior surface of the detection chamber, each of the probes being capable of forming a detectable probe:target complex with a target nucleic acid.
9. The receptacle of claim 1 , wherein the plurality of chambers further comprise a reconstitution chamber directly connected to the reagent chamber, and wherein the receptacle further comprises:
a dried reagent contained in the reagent chamber, and
a reconstitution reagent contained in the reconstitution chamber.
10. The receptacle of claim 9 , wherein the plurality of chambers further comprise a second reagent chamber directly connected to the process chamber and a second reconstitution chamber directly connected to the second reagent chamber, and wherein the receptacle further comprises:
a second dried reagent contained in the second reagent chamber, and
a second reconstitution reagent contained in the second reconstitution chamber, wherein the dried reagent and the second dried reagent include an amplification reagent and an enzyme reagent, the dried reagent and the second dried reagent being useful for performing a nucleic acid-based amplification reaction.
11. The receptacle of claim 10 , wherein at least one of the amplification and enzyme reagents comprises one or more probes, each of the probes being capable of forming a detectable probe:target complex with a product of the nucleic acid-based amplification reaction.
12. The receptacle of claim 1 , wherein the receptacle is held within a sealed container.
13. The receptacle of claim 12 , wherein the container further holds a desiccant.
14. The receptacle of claim 1 , wherein the sample inlet port is closed with a Luer connection.
15. The receptacle of claim 10 , further comprising a sample binding reagent contained in one of the plurality of chambers, wherein the sample binding reagent is adapted to bind to and immobilize an analyte present in the sample material on a solid support.
16. The receptacle of claim 1 , further comprising a rigid frame supporting the opposed members at their peripheral edges.
17. The receptacle of claim 16 , wherein the frame comprises an identifying label.
18. The receptacle of claim 16 , wherein the frame comprises a protecting tab projecting from the frame to provide an appendage for grasping the receptacle.
19. The receptacle of claim 16 , wherein the frame comprises a front frame component and a rear frame component, and wherein the peripheral edges of the opposed members are retained between the front and rear frame components.
20. The receptacle of claim 16 , wherein the sample inlet port extends through a portion of the frame, and the receptacle further includes a port cover configured to close off the sample inlet port.
21. The receptacle of claim 9 , wherein the dried reagent is a lyophilized reagent.
22. The receptacle of claim 1 , wherein each of the opposed members comprises a flexible sheet.
23. The receptacle of claim 1 comprising an alignment hole adjacent a top edge of the receptacle.
24. The receptacle of claim 5 , comprising an openable seal at the waste portal interconnecting the process chamber and the waste chamber.