Encapsulated kidney tissue
View Patent ↗Provided are therapeutic implants comprising renal tissue encapsulated within a polymer bead. Also disclosed are methods for treating a disease state in a subject comprising implanting within said subject a therapeutic implant comprising renal tissue encapsulated within a polymer bead. Also provided are methods for making a therapeutic implant comprising: providing renal tissue; mixing the renal tissue with a solution comprising a polymer, thereby forming a tissue-polymer suspension; extruding the tissue-polymer suspension into an bead-forming solution, thereby forming a therapeutic implant comprising beads of said polymer within which the renal tissue is encapsulated.
1. A therapeutic implant comprising excised and minced fragments of whole renal tissue having a size of less than 1 mm, encapsulated in the presence of a growth medium within a polymer bead that is permeable to substances secreted by said tissue.
2. The therapeutic implant according to claim 1 , wherein said renal tissue is autologous tissue, allogeneic tissue, xenogeneic tissue, or any combination thereof.
3. The therapeutic implant according to claim 1 , wherein said fragments of excised and minced whole renal tissue have a size of less than about 300 μm.
4. The therapeutic implant according to claim 1 , comprising at least about 30 mg of renal tissue.
5. The therapeutic implant according to claim 1 , wherein said polymer bead comprises alginate, hyaluronic acid, carboxymethylcellulose, polyethylene glycol, dextran, agarose, poly-L-lysine, carageenan, pectin, tragacanth gum, xanthan gum, guar gum, gum arabic, type I collagen, laminin, fibronectin, fibrin, or any combination thereof.
6. The therapeutic implant according to claim 1 , wherein said polymer bead comprises alginate and poly-L-lysine.
7. The therapeutic implant according to claim 1 , wherein said polymer bead has a diameter of about 3 mm to about 6 mm.
8. The therapeutic implant according to claim 1 , wherein said excised and minced renal tissue fragments secrete one or more hormones, prohormones, proteins, growth factors, or any combination thereof.
9. The therapeutic implant according to claim 8 , wherein said renal tissue fragments secrete one or more of erythropoietin, MCP-1, adiponectin, leptin, and MMP-2.
10. A method for making a therapeutic implant comprising:
providing fragments of excised and minced whole renal tissue having a size of less than 1 mm;
mixing said fragments of excised and minced whole renal tissue with a solution comprising a first polymer and a growth medium, thereby forming a tissue-polymer suspension;
extruding said tissue-polymer suspension into a bead-forming solution, thereby forming a therapeutic implant comprising beads of said polymer within which said fragments of excised and minced whole renal tissue and growth medium are encapsulated.
11. The method according to claim 10 , wherein said first polymer is selected from the group consisting of alginate, hyaluronic acid, carboxymethylcellulose, polyethylene glycol, dextran, agarose, poly-L-lysine, carageenan, pectin, tragacanth gum, xanthan gum, guar gum, gum arabic, type I collagen, laminin, fibronectin, fibrin, or any combination thereof.
12. The method according to claim 10 , wherein said bead-forming solution comprises a cross-linking solution.
13. The method according to claim 10 , wherein said bead-forming solution comprises an ionic solution.
14. The method according to claim 10 , wherein said bead-forming solution comprises CaCl 2 .
15. The method according to claim 10 , further comprising coating said beads with an additional polymer layer.
16. The method according to claim 10 , further comprising cross-linking said polymer beads by introducing said polymer beads into a cross-linking solution containing a second polymer.
17. The method of claim 16 , wherein said first polymer is alginate and said second polymer is poly-L-lysine.
18. The method of claim 15 , wherein said additional polymer layer comprises alginate.