Functional influenza virus like particles (VLPs)
The present invention discloses and claims virus like particles (VLPs) that express and/or contains seasonal influenza virus proteins, avian influenza virus proteins and/or influenza virus proteins from viruses with pandemic potential. The invention includes vector constructs comprising said proteins, cells comprising said constructs, formulations and vaccines comprising VLPs of the inventions. The invention also includes methods of making and administrating VLPs to vertebrates, including methods of inducing substantial immunity to either seasonal and avian influenza, or at least one symptom thereof.
1. A method of inducing substantial immunity to an influenza virus infection in a human susceptible to influenza, comprising administering to said human at least one effective dose of a vaccine, said vaccine comprising an influenza VLP, wherein said VLP comprises influenza M1, HA and NA proteins and wherein said M1 protein is from an avian influenza virus, and wherein said M1 protein is from a different strain of influenza virus than said influenza HA protein and said influenza NA protein.
2. The method of claim 1 , wherein said HA and/or NA exhibits hemagglutinin activity and/or neuraminidase activity, respectively.
3. The method of claim 1 , wherein said method comprises administering to a human said vaccine orally, intradermally, intranasally, intramusclarly, intraperitoneally, intravenously, or subcutaneously.
4. The method of claim 1 , wherein at least two effective doses of the vaccine are administered.
5. The method of claim 4 , wherein said doses are administered at least 2 weeks apart, at least 3 weeks apart, at least 4 weeks apart, at least 5 weeks apart or at least 6 weeks apart.
6. The method of claim 1 , wherein said dose comprises at least 10 μg of the influenza VLP.
7. The method of claim 1 , wherein said dose comprises from about 10 μg to 150 μg of the influenza VLP.
8. The method of claim 1 , wherein said dose comprises from about 15 μg to about 45 μg of the influenza VLP.
9. The method of claim 4 , wherein said doses comprise from about 15 μg to about 45 μg of the influenza VLP.
10. The method of claim 1 , wherein said dose comprises from about 45 μg to about 135 μg of the influenza VLP.
11. The method of claim 4 , wherein said doses comprise from about 45 μg to about 135 μg of the influenza VLP.
12. The method of claim 1 , wherein said influenza HA and NA proteins are seasonal influenza virus HA and NA proteins.
13. The method of claim 12 , wherein said seasonal influenza virus is a type A influenza virus.
14. The method of claim 12 , wherein said seasonal influenza virus is a type B influenza virus.
15. The method of claim 1 , wherein said influenza HA and NA proteins are avian influenza virus HA and NA proteins.
16. The method of claim 15 , wherein said avian influenza virus HA and NA proteins are derived from H5N1.
17. The method of claim 15 , wherein said avian influenza virus HA and NA proteins are derived from H9N2.
18. The method of claim 1 , wherein said M1 protein is derived from A/Indonesia/5/05.
19. The method of claim 1 , wherein said vaccine further comprises an adjuvant or immune stimulator.