IP Library › Patent Application 12341442
Patent Application
App. No. 12/341,442

HUMAN RNASE III AND COMPOSITIONS AND USES THEREOF

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Quick Facts
Patent No.
US None
App. No.
12/341,442
Abstract

The present invention provides polynucleotides encoding human RNase III and polypeptides encoded thereby. Methods of using said polynucleotides and polypeptides are also provided.

Claims (21)

1 - 64 . (canceled)

65 . A method comprising contacting a cell with a modified oligonucleotide wherein:

the modified oligonucleotide is 12 to 30 nucleobases in length;

the modified oligonucleotide is complementary to nucleobases 3051 to 4004 of SEQ ID NO: 1 or to nucleobases 4197 to 4397 of SEQ ID NO: 1.

66 . The method of claim 65 wherein the modified oligonucleotide is complementary to nucleobases 3051 to 4004 of SEQ ID NO: 1.

67 . The method of claim 66 wherein in the modified oligonucleotide comprises the nucleobase sequence of SEQ ID NO: 8 or 9.

68 . The method of claim 65 wherein the modified oligonucleotide is complementary to nucleobases 4197 to 4397 of SEQ ID NO: 1.

69 . The method of claim 68 wherein the modified oligonucleotide comprises the nucleobase sequence of SEQ ID NO: 12, 13, 14, or 15.

70 . The method of claim 65 , wherein the modified oligonucleotide comprises at least one nucleoside comprising a modified sugar.

71 . The method of claim 70 , wherein at least one modified nucleoside comprises a 2′-O-methoxyethyl.

72 . The method of claim 65 wherein the modified oligonucleotide comprises at least one modified internucleoside linkage.

73 . The method of claim 65 , wherein the modified oligonucleotide comprises:

a gap segment consisting of linked deoxynucleosides;

a 5′ wing segment consisting of linked modified nucleosides; and

a 3′ wing segment consisting of linked modified nucleosides;

wherein the gap segment is positioned between the 5′ wing segment and the 3′ wing segment and wherein each modified nucleoside of each wing segment comprises a modified sugar.

74 . The method of claim 73 , wherein each modified sugar comprises a 2′-modification.

75 . The method of claim 74 , wherein each 2′-modification is a 2′-O-methoxyethyl modification.

76 . The method of claim 65 , wherein the cell is in an animal.

77 . The method of claim 76 , wherein the animal is a mammal.

78 . The method of claim 77 , wherein the animal is a human.