Mussel adhesive protein derived vectors for gene delivery
View Patent ↗The present invention is related to a composition and a method for delivering a nucleic acid into a cell. The invention also provides a biocompatible and biodegradable gene delivery composition and methods of use and making thereof.
1. A method for delivering a nucleic acid-fusion protein complex into a eukaryotic cell, said method comprising
contacting a eukaryotic cell with a complex comprising a nucleic acid and a fusion protein, wherein the fusion protein comprises the formula X-(FP-5)-X, where each X is the same and is chosen from the group consisting of FP-1, FP-2, FP-3, FP-4, FP-6 and SEQ ID NO: 2, and
wherein subsequent to the contacting step, the complex enters the eukaryotic cell.
2. The method of claim 1 , wherein the gene-delivering peptide further comprises nuclear localization signal peptide or a protein transduction domain.
3. The method of claim 1 , wherein the gene-delivering peptide further comprises a purification tag.
4. The method of claim 1 , wherein X=FP-1.
5. The method of claim 4 , wherein the fusion protein comprises SEQ ID NO:3.
6. The method of claim 1 , wherein X=FP-3.
7. The method of claim 6 , wherein the fusion protein comprises SEQ ID NO:5.
8. The method of claim 1 , wherein the cells are contained in a solution containing a calcium compound.
9. The method of claim 1 , wherein the weight ratio of the fusion protein to the nucleic acid is 1:1 to 100:1.
10. The method of claim 1 , wherein the nucleic acid is one selected from the group consisting of a genomic DNA, an RNA, an oligonucleotide, and an antisense nucleic acid.