Nucleic acid molecules encoding prostaglandin receptor proteins
View Patent ↗Described herein is a novel member of the prostanoid receptor family, a guinea pig prostaglandin D2 receptor. Described are the receptor, the nucleic acid that encodes it, and various uses for both.
1. An isolated nucleic acid molecule comprising the nucleic acid sequence of SEQ ID NO:1.
2. The isolated nucleic acid molecule of claim 1 , wherein the nucleic acid encodes the polypeptide of SEQ ID NO:2.
3. The isolated nucleic acid molecule of claim 1 , further comprising a detectable label.
4. The isolated nucleic acid molecule of claim 3 , wherein the detectable label comprises an enzyme, a radioactive isotope, or a chemical which fluoresces.
5. The isolated nucleic acid molecule of claim 1 , wherein the nucleic acid sequence is selected from the group consisting of RNA, synthetic RNA, genomic DNA, synthetic DNA and cDNA.
6. An isolated nucleic acid molecule comprising a nucleic acid sequence that hybridizes to the nucleic acid sequence of SEQ ID NO: 1, wherein the hybridization occurs in 6×SSC at about 45° C., followed by at least one wash in 0.2×SSC, 0.1% SDS at about 50-65° C. and wherein a complement of said nucleic acid encodes a polypeptide comprising an amino acid sequence of SEQ ID NO:2.
7. An isolated nucleic acid molecule comprising a nucleic acid sequence that hybridizes to the nucleic acid sequence of SEQ ID NO:1, wherein the hybridization occurs in 6×SSC at about 45° C., followed by at least one wash in 0.2×SSC, 0.1% SDS at about 50-65° C. and wherein a complement of said nucleic acid encodes a polypeptide comprising an amino acid sequence at least 95% identical to SEQ ID NO:2 and which binds a prostanoid.
8. An isolated nucleic acid molecule comprising a nucleic acid that encodes a polypeptide having the amino acid sequence of SEQ ID NO:2.
9. The nucleic acid molecule of claim 8 , where, as a result of the degeneracy of the genetic code, the nucleic acid differs from the nucleic acid of SEQ ID NO:1.
10. An isolated nucleic acid molecule, wherein the nucleic acid is at least 95% identical to the nucleic acid of SEQ ID NO:1 and encodes a polypeptide comprising an amino acid sequence of SEQ ID NO:2.
11. An isolated nucleic acid molecule comprising a nucleic acid sequence that encodes a recombinant polypeptide wherein the amino acid sequence is at least 95% identical to SEQ ID NO:2 and which binds a prostanoid.
12. An expression vector comprising the isolated nucleic acid molecule of claim 1 operatively associated with an expression control element.
13. The expression vector of claim 12 , wherein the expression control element is selected from the group consisting of a constitutive regulatory sequence, a cell-specific regulatory sequence, and an inducible regulatory sequence.
14. The expression vector of claim 13 , wherein the expression control element is a promoter comprising an immediate early promoter of hCMV, an early promoter of SV40, an early promoter of adenovirus, an early promoter of vaccinia, an early promoter of polyoma, a late promoter of SV40, a late promoter of adenovirus, a late promoter of vaccinia, a late promoter of polyoma, a lac system, a trp system, a TAC system, a TRC system, a major operator and promoter region of phage lambda, a control region of fd coat protein, 3-phosphoglycerate kinase promoter, acid phosphatase promoter, or a promoter of yeast α mating factor.
15. A cultured host cell transfected with the expression vector of claim 12 .
16. The cultured host cell of claim 15 , wherein the host cell is selected from the group consisting of a prokaryotic cell and a eukaryotic cell.
17. The cultured host cell of claim 16 , wherein the host cell is selected from the group consisting of E. coli, Pseudonomas, Bacillus, Strepomyces , yeast, CHO, R1.1, B-W, L-M, COS1, COS7, BSC1, BSC40, BMT10 and Sf9 cells.
18. An expression vector comprising said complement of claim 6 or 7 operatively associated with an expression control element.
19. A cultured host cell transfected with the expression vector of claim 18 .
20. An expression vector comprising the isolated nucleic acid molecule of claim 10 or 11 operatively associated with an expression control element.
21. A cultured host cell transfected with the expression vector of claim 20 .