Determination of sFlt-1:angiogenic factor complex
View Patent ↗Methods for determining the presence or amount of a complex comprising a first and second molecular entity are provided, preferably an sFlt-1:PlGF complex. A determination of the presence or amount of the complex can be used in methods for predicting, detecting, monitoring a disease, or guiding therapy in respect to a disease such as vascular, vascular-related disease, cardiac, cardiac-related disease, cancer, cancer-related disease, preeclampsia, and preeclampsia-related disease. Determining sFlt-1:angiogenic factor complex is particularly useful for predicting and detecting preeclampsia in early stages of gestation and in stages of the disease where clinical evaluation may be uninformative.
1. A composition for determining the presence or amount of sFlt-1:PlGF complex, the composition consisting of a single capture agent and a single detector agent, wherein the single capture agent and the single detector agent consist of:
a) a single capture agent that binds to PlGF and a single detector agent that binds to one or both of sFlt-1 and sFlt-1:PlGF complex; or
b) a single capture agent that binds to sFlt-1 and a single detector agent that binds to one or both of PlGF and sFlt-1:PlGF complex; or
c) a single capture agent that binds to sFlt-1:PlGF complex and a single detector agent that binds to sFlt-1:PlGF complex, wherein the single capture agent and the single detector agent are capable of being bound simultaneously to the complex; or
d) a single capture agent that binds to sFlt-1:PlGF complex and a single detector agent that binds to one or both of PlGF and sFlt-1.
2. The composition of claim 1 wherein PlGF is PlGF-1, PlGF-2, or PlGF-3.
3. The composition of claim 1 wherein one or both of capture agent and detector agent are polyclonal antibodies.
4. The composition of claim 1 wherein one or both of capture agent and detector agent are monoclonal antibodies.
5. The composition of claim 1 wherein one of the capture agent and detector agent is a monoclonal antibody and the other of the capture agent and detector agent is a polyclonal antibody.