Peptides for discrimination of prions
View Patent ↗The aim of the present invention is to provide a non-intrusive way to isolate, concentrate and monitor the TSE disease-related pathogenic prion protein. The invention described several peptides and their ability to capture PrP Sc from brain homogenate of prion disease infected animal and human. These eight peptides do not capture cellular prion protein from individual with no prion disease.
1. A method for detection of PrP Sc comprising:
first contacting a sample with a peptide consisting of an amino acid sequence selected from the group consisting of: SEQ ID NO:1: WQPPRARI; SEQ ID NO:2: NWCKRGRKQCKTH; SEQ ID NO:3: NYKKPKL; SEQ ID NO:5: YRGYRGYRGYRG; SEQ ID NO:6: YRGRYGYKGKYGYRG; SEQ ID NO:7: AQKKDGKKRKRSRKESYSIYV; SEQ ID NO:8: ARTKQTARKSTGGKSPRKQLA; and SEQ ID NO:9: SGRGKGGKGLGKGGAKRHRKVLR; and
then detecting PrP Sc with a labeled prion specific antibody, wherein the peptide specifically captures PrP Sc and the labeled prion specific antibody detects the captured PrP Sc .
2. A method for detection of PrP Sc comprising:
first contacting a sample with a prion specific antibody; and
then detecting PrP Sc with a labeled peptide consisting of an amino acid sequence selected from the group consisting of: SEQ ID NO:1: WQPPRARI; SEQ ID NO:2: NWCKRGRKQCKTH; SEQ ID NO:3: NYKKPKL; SEQ ID NO:5: YRGYRGYRGYRG; SEQ ID NO:6: YRGRYGYKGKYGYRG; SEQ ID NO:7: AQKKDGKKRKRSRKESYSIYV; SEQ ID NO:8: ARTKQTARKSTGGKSPRKQLA; and SEQ ID NO:9: SGRGKGGKGLGKGGAKRHRKVLR; wherein the prion specific antibody captures PrP C and PrP Sc and the labeled peptide specifically detects the captured PrP Sc .
3. An immunoassay for detecting PrP Sc comprising:
providing a solid support having bound thereto a peptide consisting of an amino acid sequence selected from the group consisting of: SEQ ID NO:1: WQPPRARI; SEQ ID NO:2: NWCKRGRKQCKTH; SEQ ID NO:3: NYKKPKL; SEQ ID NO:5: YRGYRGYRGYRG; SEQ ID NO:6: YRGRYGYKGKYGYRG; SEQ ID NO:7: AQKKDGKKRKRSRKESYSIYV; SEQ ID NO:8: ARTKQTARKSTGGKSPRKQLA; and SEQ ID NO:9: SGRGKGGKGLGKGGAKRHRKVLR;
contacting the solid support with a sample;
washing the support to remove any unbound sample;
contacting the solid support with a labeled prion specific antibody; and
carrying out a detection step to determine if PrP Sc are bound to the solid support, wherein the peptide specifically captures PrP Sc and the labeled prion specific antibody detects the captured PrP Sc .
4. An immunoassay for detecting PrP Sc comprising:
providing a solid support having bound thereto a prion specific antibody;
contacting the solid support with a sample;
washing the support to remove any unbound sample;
contacting the solid support with a labeled peptide consisting of an amino acid sequence selected from the group consisting of SEQ ID NO:1: WQPPRARI; SEQ ID NO:2: NWCKRGRKQCKTH; SEQ ID NO:3: NYKKPKL; SEQ ID NO:5: YRGYRGYRGYRG; SEQ ID NO:6: YRGRYGYKGKYGYRG; SEQ ID NO:7: AQKKDGKKRKRSRKESYSIYV; SEQ ID NO:8: ARTKQTARKSTGGKSPRKQLA; and SEQ ID NO:9: SGRGKGGKGLGKGGAKRHRKVLR; and
carrying out a detection step to determine if PrP Sc are bound to the solid support, wherein the prison specific antibody captures PrP C and PrP Sc and the labeled peptide specifically detects the captured PrP Sc .