HIGH-THROUGHPUT SCREENING OF METABOLIC DISORDERS USING A LASER DESORPTION ION SOURCE COUPLED TO A MASS ANALYZER
Methods and kits for high throughput screening and analysis of metabolic disorders using a laser desorption ion source coupled to a mass analyzer (for example, MALDI) are provided. The metabolic disorders can be amino acid, organic acid or fatty acid oxidation disorders. A panel of disorders can be analyzed at high speeds. The methods and kits are particularly useful for newborn screening (NBS) of metabolic disorders.
1 . A method for analyzing a biological sample for a metabolic disorder, comprising:
(a) providing the biological sample; and
(b) analyzing the sample by a laser desorption ion source coupled to a mass analyzer.
2 . The method of claim 1 wherein the step of analyzing comprises matrix assisted laser desorption ionization (MALDI) mass spectrometry.
3 . The method of claim 1 further comprising collisionally damping/cooling the ions generated by the ion source with a damping gas.
4 . The method of claim 3 wherein the step of collisionally damping/cooling is orthogonal MALDI (oMALDI).
5 . The method of claim 1 wherein the metabolic disorder is a newborn metabolic disorder.
6 . The method of claim 1 wherein the metabolic disorder is selected from the group consisting of amino acid disorder, fatty acid oxidation disorder, and organic acid disorder.
7 . The method of claim 1 wherein the disorder is selected from the group consisting of phenylketonuria (PKU), hyperphenylalaninemias, maple syrup urine disease (MSUD), homocystinuria, citrullinemia (types I and II), argininemia, argininosuccinic acidemia (ASA), tyrosinemia (types I and II), homocitrullinuria, hyperornithinemia, hyperammonemia (HHH), methionine adenosyltransferase (MAT) deficiency, biopterin deficiencies, prolinemia, hypermethioninemia, gyrate atrophy of choroid and retina, medium chain acyl-CoA dehydrogenase (MCAD) deficiency, very long chain acyl-CoA dehydrogenase (VLCAD) deficiency, short chain acyl-CoA dehydrogenase (SCAD) deficiency, multiple acyl-CoA dehydrogenase (MAD) deficiency, long chain 3-hydroxyacyl-CoA dehydrogenase (LCHAD) deficiency, medium/short chain L-3-hydroxyacyl-CoA dehydrogenase (M/SCHAD) deficiency, trifunctional protein deficiency (TFP), carnitine palmitoyltransferase deficiencies of types I and II (CPT-I, CPT-II), carnitine-acylcarnitine translocase (CACT) deficiency, carnitine transporter deficiency, carnitine uptake defect, short chain 3-ketoacyl-CoA thiolase (SKAT) deficiency, medium chain 3-ketoacyl-CoA thiolase (MCKAT) deficiency, 2,4-dienoyl-CoA reductase deficiency, glutaric acidemia type II (GA-II), 3-methylcrotonyl-CoA carboxylase (3-MCC) deficiency, glutaric acidemia type I (GA-I), methylmalonic acidemias (MMA), propionic acidemia (PA), isovaleric acidemia (IVA), malonic aciduria (MA), multiple carboxylase deficiency (MCD), 2-methyl-3-hydroxybutyrl-CoA Dehydrogenase (MHBD) deficiency, 3-hydroxy-3-methylglutaryl-CoA lyase (HMG) deficiency, 2-methylbutyryl-CoA dehydrogenase (2 MBCD) deficiency, 3-methylglutaconic acidurias (MGA), isobutyryl-CoA dehydrogenase (IBD) deficiency, beta-ketothiolase deficiency (BKT), and ethylmalonic encephalopathy (EE).
8 . The method of claim 1 wherein the step of providing the biological sample comprises derivatizing the sample and spotting on a target surface.
9 . The method of claim 8 wherein derivatizing the sample comprises derivatization to butyl esteric form.
10 . The method of claim 2 wherein the step of providing the biological sample comprises mixing the biological sample approximately 1:1 with CHCA.
11 . The method of claim 1 wherein the biological sample is an underivatized amino acid or acylcarnitine and step (a) comprises (a) mixing the underivatized sample with UV substance and (b) spotting the underivatized sample on a target surface.
12 . The method of claim 1 wherein the step of analyzing the sample comprises a tandem mass spectrometer, a triple quadrupole mass analyzer or an ion trap mass spectrometer.
13 . The method of claim 12 further comprising multiple reaction monitoring.
14 . The method of claim 1 further comprising a step of liquid chromatography prior to analyzing the sample by mass spectrometry.
15 . The method of claim 1 wherein the biological sample is chosen from whole blood, blood components, frozen blood, blood spots, serum, plasma, physiological fluid, physiological tissue, inner cheek swabs, hair, cerebral spinal fluid, vitreous humor, or amniotic fluid.
16 . The method of claim 1 wherein the step of analyzing comprises analysis in the positive ion mode or the negative ion mode.
17 . The method of claim 1 wherein the step of analyzing the sample comprises analysis of a panel of biological samples.
18 . The method of claim 17 wherein the analysis is done by a discrete mode of operation, a rastering mode of operation, or by a pattern raster mode of operation.
19 . The method of claim 17 wherein the step of providing the biological sample comprises spotting the biological sample on a target surface to produce a panel of biological samples, and wherein at least one spot comprises a biological sample that is analyzed for at least two biological disorders simultaneously.
20 . A method for multiplexed analysis of a panel of biological samples for biological disorders comprising:
(a) providing the panel of biological samples;
(b) spotting the panel of biological samples on a target surface; and
(c) analyzing the panel of biological samples by a laser desorption ion source coupled to a mass analyzer.
21 . A kit for the analysis of a metabolic disorder, comprising one or more of:
(i) reagents for collection, preparation and/or analysis of biological samples comprising or suspected of comprising analytes of metabolic disorders for analysis by laser desorption ion source coupled to a mass analyzer; and
(ii) instructions for the collection, preparation and/or analysis of samples for analysis by laser desorption ion source coupled to a mass analyzer.
22 . The kit of claim 21 wherein the metabolic disorder is a newborn metabolic disorder.
23 . The kit of claim 21 for the analysis of a panel of metabolic disorders.