Ion fragmentation in mass spectrometry
View Patent ↗In a tandem mass spectrometer using a collision cell for ion fragmentation, the upper limit of the collision energy required for collision induced dissociation (CID) can be extended without reaching or going beyond the upper electrical discharge limit of the system components. The present teachings describe a method of lifting the potential energy of ions to a predetermined level sufficient for CID fragmentation while satisfying a discharge free condition. The present teaching also describes a method of lifting the potential energy of the fragment ions after CID fragmentation so that the product ions have sufficient energy for mass analysis.
1. A method of performing tandem mass spectrometry comprising:
providing a high pressure ion guide configured for accepting ions;
storing the ions in the high pressure ion guide;
raising the potential energy of the stored ions so that the stored ions have a predetermined energy level for collisional induced dissociation;
releasing the stored ions from the high pressure ion guide and transmitting precursor ions into a collision cell, the collision cell having a background gas;
colliding the precursor ions with the background gas and dissociating the precursor ions to produce product ions; and
analyzing the product ions.
2. The method of claim 1 further comprising mass selecting precursor ions from the released stored ions for transmission into the collision cell.
3. The method of claim 2 further comprising operating the high pressure ion guide at near ground potential while storing the ions.
4. The method of claim 3 wherein raising the potential energy of the stored ions is by increasing a DC offset voltage of the high pressure ion guide.
5. The method of claim 4 wherein the product ions are analyzed with a time-of-flight analyzer.
6. A method of performing tandem mass spectrometry comprising:
providing a high pressure ion guide configured for accepting ions and providing a collision cell configured for storing product ions;
accelerating the ions from the high pressure ion guide and transmitting precursor ions into the collision cell, the collision cell having a background gas;
colliding the precursor ions with the background gas to produce product ions;
storing the product ions in the collision cell;
raising the potential energy of the product ions to a predetermined level sufficient for releasing the product ions from the collision cell; and
analyzing the product ions.
7. The method of claim 6 further comprising mass selecting precursor ions from the group of ions for transmission into the collision cell.
8. The method of claim 7 wherein the high pressure ion guide configuration comprise of operating with a positive DC offset voltage for accepting the ions and the collision cell configuration comprise of operating with a negative DC offset voltage for storing the product ions.
9. The method of claim 8 wherein the product ions are analyzed with a time-of-flight analyzer.