Novel Biodegradable Elastomeric Scaffold for Tissue Engineering and Light Scattering Fingerprinting Methods for Testing the Same
The present invention is directed to a novel biocompatible polymer that may be used in tissue engineering. More specifically, the specification describes methods and compositions for making and using a citric acid copolymers.
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8 . A substrate for use in tissue engineering comprising a citric acid polymer of claim 15 formulated into a cell culture substrate.
9 . The substrate of claim 15 , wherein said substrate further comprises a surface modification.
10 . The substrate of claim 15 , wherein said polymer is biodegradeable.
11 . A method of producing engineered tissue, comprising:
a. providing a biodegradable citric acid polymer of any of claim 15 ; and
b. culturing cells of said tissue on the scaffold of part (a).
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13 . The method of claim 11 , wherein said cells are selected from the group consisting of endothelial cells, ligament tissue, muscle cells, bone cells, cartilage cells.
14 . The method of claim 11 , wherein said engineered tissue is produced in a bioreactor.
15 . A citric acid polyester comprising a reaction product of monomers A, B, and C,
wherein A is a linear aliphatic dihydroxy monomer, B is citric acid, and C is a linear aliphatic dihydroxy monomer, and A and C are different linear diols, and the polyester comprises four or more citric acid monomers.
16 . The polyester of claim 15 , wherein A and C are each a linear diol comprising between about 2 and about 20 carbons.
17 . The polyester of claim 15 , wherein A is 1,8-octanediol.
18 . The polyester of claim 17 , when B comprises 1,10-decanediol, tetradeca-2,12-diene- 1,14-diol, a polyethylene oxide, N-methyldiethanolamine, 1,6-hexanediol, 1,12-dodecanediol, or mixtures thereof.
19 . The polyester of claim 18 further comprising glycerol.