Methods and kits for extraction of DNA
Methods and materials are disclosed for use in recovering a biopolymer from a solution. In particular, the invention provides methods for extraction and isolation of nucleic acids from biological materials. The nucleic acids can be separated by forming a stable complex with soluble polysaccharide polymers and magnetic particles, in the presence of detergents and solvent. When the particles are magnetically separated out of the solution, the nucleic acids are separated with them. The nucleic acids can subsequently be released and separated from the particles. The nucleic acid preparation is useful for achieving efficient and accurate results in downstream molecular techniques such as quantification, identification of the source of the nucleic acids, and genotyping.
1. A method comprising:
(a) contacting a composition comprising a nucleic acid with a polymer, a detergent, isopropanol and dextran encased particles thereby forming a blend, wherein the polymer is dextran and the detergent is lauroyl sarcosinate;
(b) isolating the dextran encased particles from the blend;
(c) and
(d) contacting the recovered dextran encased particles with a solution comprising ethanol and thereby isolating the nucleic acid from the composition.
2. The method of claim 1 , wherein the nucleic acid is derived from a sample of biological material comprising one or more of blood, blood stain, saliva, saliva stain, buccal cells, buccal swab, semen, or semen stain.
3. The method of claim 1 , wherein the dextran is in the range of 1-5 mg/ml.
4. The method of claim 1 , wherein the dextran has a molecular weight of 5,000,000 to 40,000,000.
5. The method of claim 1 , wherein the lauroyl sarconsinate is in the range of about 5-15%.
6. The method of claim 1 , wherein the dextran encased particles are in the range of about 2-10 mg/ml.