Genetically stable attenuated polioviruses comprising multiple mutations in domain V of the 5′ noncoding region
View Patent ↗The invention provides an attenuated poliovirus which does not have a base pair mismatch in stem (a) or (b) of domain V of the 5′ non-coding region of its genome, wherein at least seven of the base pairs in stems (a) and (b) are U-A or A-U base pairs.
1. A poliovirus which does not have a base pair mismatch in stem (a) or (b) of domain V of the 5′ non-coding region of its genome, wherein at least seven of the base pairs in stems (a) and (b) are U-A or A-U base pairs.
2. A poliovirus according to claim 1 , wherein at least five of the base pairs in stem (b) are U-A or A-U base pairs.
3. A poliovirus according to claim 1 , wherein six of the base pairs in stem (b) are U-A or A-U base pairs.
4. A poliovirus according to claim 1 , wherein seven of the base pairs in stem (b) are U-A or A-U base pairs.
5. A poliovirus according to claim 1 , wherein two of the base pairs in stem (a) are U-A or A-U base pairs.
6. A poliovirus according to claim 1 , wherein three of the base pairs in stem (a) are U-A or A-U base pairs.
7. A poliovirus according to claim 1 , which comprises domain V of the 5′ non-coding region of poliovirus type 3 in which a U-A base pair is present at position 472-537 in stem (a) and at positions 478-533, 480-531 and 481-530 in stem (b).
8. A poliovirus according to claim 7 , in which an A-U base pair is present at position 482-529 in stem (b).
9. A poliovirus according to claim 7 , in which an A-U base pair is present at position 477-534 in stem (b).
10. A poliovirus according to claim 1 which is inactivated.