In vivo transfection in avians
View Patent ↗The present invention provides for methods of producing transgenic avians which may include delivering a heterologous nucleic acid to oviduct tissue of an avian wherein the nucleic acid enters a cell of the oviduct tissue and is expressed.
1. A method of transfecting an avian comprising:
delivering a nucleic acid solution under pressure to oviduct tissue of an avian by injecting the nucleic acid solution into an artery selected from the group consisting of an anterior oviductal artery; a middle oviductal artery; a superior uterine artery; inferior oviductal artery; a middle uterine artery; and an inferior uterine artery wherein the nucleic acid sequence enters a cell of oviduct tissue and is expressed.
2. The method of claim 1 wherein the avian is selected from the group consisting of chicken, quail, turkey, duck, goose, pheasant, parrot, finches, hawk, crow, ratite, ostrich, emu, and cassowary.
3. The method of claim 1 wherein the avian is a chicken.
4. The method of claim 1 wherein the nucleic acid sequence is DNA.
5. The method of claim 1 wherein the cell is a tubular gland cell.
6. The method of claim 1 wherein a polypeptide encoded by a coding sequence of the nucleic acid sequence is present in egg white produced by the avian.
7. The method of claim 6 wherein the polypeptide is a pharmaceutical protein.
8. The method of claim 1 wherein the polypeptide is an antibody.
9. The method of claim 1 wherein the polyeptide is a cytokine.