Compositions, splice variants and methods relating to ovarian specific genes and proteins
The present invention relates to newly identified nucleic acid molecules and polypeptides present in normal and neoplastic ovarian cells, including fragments, variants and derivatives of the nucleic acids and polypeptides. The present invention also relates to antibodies to the polypeptides of the invention, as well as agonists and antagonists of the polypeptides of the invention. The invention also relates to compositions containing the nucleic acid molecules, polypeptides, antibodies, agonists and antagonists of the invention and methods for the use of these compositions. These uses include identifying, diagnosing, monitoring, staging, imaging and treating ovarian cancer and non-cancerous disease states in ovarian, identifying ovarian tissue, monitoring and identifying and/or designing agonists and antagonists of polypeptides of the invention. The uses also include gene therapy, production of transgenic animals and cells, and production of engineered ovarian tissue for treatment and research.
1. A method for determining the presence of an ovarian specific protein comprising a polypeptide comprising an amino acid sequence with at least 95% sequence identity to SEQ ID NO:254 in a sample, comprising the steps of:
(a) contacting the sample with a suitable reagent which selectively interacts with the ovarian specific protein under conditions in which the reagent will selectively interact with the ovarian specific protein; and
(b) detecting the interaction of the reagent with an ovarian specific protein in the sample, wherein the detection of binding indicates the presence of an ovarian specific protein in the sample.
2. The method of claim 1 wherein the suitable reagent which selectively interacts with the ovarian specific protein is an antibody or fragment thereof that specifically binds to a polypeptide comprising an amino acid sequence with at least 95% sequence identity to SEQ ID NO:254 or a peptide thereof.
3. The method of claim 2 wherein the antibody or fragment thereof binds a peptide of SEQ ID NO: 254, wherein said peptide comprises amino acids 1-341, 191-198, 251-261, 4-23, 217-227, 321-331, 298-304, 341-366, 208-215, 267-275, 180-185, 285-294, 92-115, 121-146, 71-77, 212-214, 203-206, 154-157, 247-249, 365-367, 94-96, 151-156, 304-306, 48-51, 10-15, 188-193, 237-239, 150-153, 179-184, 227-233, 70-72, 66-68, 274-277, 203-205, 39-44, 81-84, 128-133, 276-278, 162-167, 187-190, 199-204, 210-213, 339-344, 279-282, 142-211, 172-215, 171-199 or 26-101 of SEQ ID NO: 254.
4. The method of claim 3 wherein the antibody or fragment thereof binds the peptide comprising amino acids 1-341 of SEQ ID NO: 254.
5. The method of claim 3 wherein the antibody or fragment thereof binds the peptide comprising amino acids 26-101 of SEQ ID NO: 254.
6. The method of claim 2 wherein the antibody or fragment thereof binds a peptide of SEQ ID NO: 254, wherein said peptide comprises a post translational modification, motif, or domain which is a thyroglobulin domain, TY domain, myristyl site, glycosylation site, pkc phospho site, ck2 phospho site, tyr phospho site or amidation site.
7. The method of claim 2 wherein the antibody or fragment is a monoclonal, polyclonal, human, humanized or chimeric antibody or fragment thereof.
8. The method of claim 2 wherein the antibody or fragment thereof is labeled.
9. A method for diagnosing or monitoring the presence and metastases of ovarian cancer in a patient, comprising the steps of:
(a) determining an amount of:
a polypeptide comprising an amino acid sequence with at least 95% sequence identity to SEQ ID NO:254;
and
(b) comparing the amount of the determined polypeptide in the sample of the patient to the amount of the ovarian specific marker in a normal control; wherein a difference in the amount of the polypeptide in the sample compared to the amount of the polypeptide in the normal control is associated with the presence of ovarian cancer.
10. The method of claim 9 wherein the amount of a polypeptide comprising an amino acid sequence with at least 95% sequence identity to SEQ ID NO:254 is determined with an antibody or fragment thereof that specifically binds to a polypeptide comprising an amino acid sequence with at least 95% sequence identity to SEQ ID NO:254 or a peptide thereof.
11. The method of claim 10 wherein the antibody or fragment thereof binds a peptide of SEQ ID NO: 254, wherein said peptide comprises amino acids 1-341, 191-198, 251-261, 4-23, 217-227, 321-331, 298-304, 341-366, 208-215, 267-275, 180-185, 285-294, 92-115, 121-146, 71-77, 212-214, 203-206, 154-157, 247-249, 365-367, 94-96, 151-156, 304-306, 48-51, 10-15, 188-193, 237-239, 150-153, 179-184, 227-233, 70-72, 66-68, 274-277, 203-205, 39-44, 81-84, 128-133, 276-278, 162-167, 187-190, 199-204, 210-213, 339-344, 279-282, 142-211, 172-215, 171-199 or 26-101 of SEQ ID NO: 254.
12. The method of claim 11 wherein the antibody or fragment thereof binds the peptide comprising amino acids 1-341 of SEQ ID NO: 254.
13. The method of claim 11 wherein the antibody or fragment thereof binds the peptide comprising amino acids 26-101 of SEQ ID NO: 254.
14. The method of claim 10 wherein the antibody or fragment thereof binds a peptide of SEQ ID NO: 254, wherein said peptide comprises a post translational modification, motif, or domain which is a thyroglobulin domain, TY domain, myristyl site, glycosylation site, pkc phospho site, ck2 phospho site, tyr phospho site or amidation site.
15. The method of claim 10 wherein the antibody or fragment thereof is a monoclonal, polyclonal, human, humanized or chimeric antibody or fragment thereof.
16. The method of claim 10 wherein the antibody or fragment thereof is labeled.