Novel receptors
The invention provides isolated nucleic acid and amino acid sequences of novel receptors, antibodies to such receptors, methods of detecting such nucleic acids and receptors, and methods of screening for modulators of the receptors.
1 . A method of treating a patient who has, or is at risk for, multiple sclerosis, which comprises administering a TGR343 modulator.
2 . The method of claim 1 , wherein the TGR343 modulator is an isolated antibody that selectively binds to a TGR343 protein which comprises the amino acid sequence of SEQ ID NO:10.
3 . The method of claim 2 , wherein the antibody is selected from the group consisting of a fragment of an antibody, a monoclonal antibody, and a polyclonal antibody.
4 . The method of claim 3 , wherein the antibody is a fragment selected from the group consisting of a Fab fragment, a Fab′ fragment, and a F(ab′).sub.2 fragment.
5 . The method of claim 2 , wherein the antibody is recombinantly produced.
6 . A method of detecting TGR343 in a sample comprising:
a. isolating a biological sample;
b. contacting the biological sample with an antibody which selectively binds to a TGR343 protein which comprises the amino acid sequence of SEQ ID NO:10; and
c. detecting selective association of the antibody with the biological sample.
7 . The method of claim 6 wherein the detection is by immunoassay.
8 . The method of claim 6 wherein the biological sample is a tissue.
9 . The method of claim 6 wherein the biological sample is from a patient suspected to have multiple sclerosis.
10 . The method of claim 6 wherein the antibody is bound to a solid support.
11 . The method of claim 6 wherein the antibody is selected from the group consisting of a monoclonal antibody, a polyclonal antibody, an Fab fragment, an Fab′ fragment, or an F(ab′).sub.2 fragment.
12 . A purified molecule selected from the group consisting of: a nucleic acid molecule encoding a polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, and SEQ ID NO:12; a nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:9, and SEQ ID NO:11; and a polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, and SEQ ID NO:12.