Method of screening for compounds that disaggregate amyloid aggregates
View Patent ↗A peptide comprising at least 5 amino acid residues and less than 15 amino acid residues, the peptide including an amino acid sequence as set forth in SEQ ID NO: 7 as well as pharmaceutical compositions, kits and methods for diagnosing and treating amyloid associated diseases.
1. A method of screening for and identifying compounds that disaggregate amyloid aggregates, the method comprising:
(a) contacting test compounds to be screened, with an amyloid aggregate formed by causing self-aggregation of a labeled peptide having at least 5 amino acid residues and less than 15 amino acid residues, said peptide comprising an amino acid sequence as set forth in SEQ ID NO: 7 that is capable of self-aggregating into an amyloid aggregate; and
(b) monitoring displacement of said labeled peptide from the amyloid aggregate by each test compound; and
(c) identifying any compound shown in said monitoring step to cause displacement of said labeled peptide, thereby causing disaggregation of the amyloid aggregate.
2. The method of claim 1 , wherein said peptide further comprises at least two serine residues attached to a C-terminus thereof.
3. The method of claim 1 , wherein said peptide is a linear peptide.
4. The method of claim 1 , wherein said peptide is a cyclic peptide.
5. The method of claim 1 , wherein said sequence as set forth in SEQ ID NO: 7 is the sequence of SEQ ID NO:13.
6. The method of claim 1 , wherein said sequence as set forth in SEQ ID NO: 7 is the sequence of SEQ ID NO:4.
7. The method of claim 1 , wherein said sequence as set forth in SEQ ID NO: 7 is the sequence of SEQ ID NO:12.