Postpartum cells derived from umbilical cord tissue, and methods of making and using the same
View Patent ↗Cells derived from human umbilical cords are disclosed along with methods for their therapeutic use. Isolation techniques, culture methods and detailed characterization of the cells with respect to their cell surface markers, gene expression, and their secretion of trophic factors are described.
1. A cell culture comprising an isolated cell obtained from mammalian umbilical cord tissue substantially free of blood cultured in a culture medium, wherein said isolated cell is capable of self-renewal and expansion of its numbers in culture, expresses CD10, CD13, CD44, CD73, CD90, PDGFr-alpha, HLA-A, HLA-B, and HLA-C, does not express CD31, CD34, CD45, CD117, CD141, HLA-DR, HLA-DP and HLA-DQ, has increased expression, relative to a human fibroblast, mesenchymal stem cell, or iliac crest bone marrow cell, of a gene for interleukin 8, reticulon 1 and chemokine (C-X-C motif) ligand 3, and produces granulocyte chemotactic protein-2 (GCP-2), and wherein the culture medium is chemically-defined medium or medium with added mammalian serum.
2. The cell culture of claim 1 , further comprising L-valine.
3. The cell culture of claim 1 , wherein the isolated cell secretes MCP-1, IL-6, IL-8, GCP-2, HGF, KGF, FGF, HB-EGF, BDNF, TPO, MIP1b, RANTES, and TIMP1, and does not secrete SDF-1alpha, TGF-beta2, ANG2, PDGFbb, MIP1a, and VEGF.
4. A cell culture comprising an isolated cell obtained from mammalian umbilical cord tissue substantially free of blood cultured in a culture medium, wherein said cell is capable of self-renewal and expansion and wherein said cell has the characteristics of cells deposited with ATCC under Accession No. PTA-6067, and wherein the culture medium is chemically-defined medium or medium with added mammalian serum.
5. A cell culture comprising an isolated cell obtained from mammalian umbilical cord tissue substantially free of blood cultured in a culture medium, wherein said cell is capable of self-renewal and expansion and wherein said cell has the characteristics of cells deposited with ATCC under Accession No. PTA-6068, and wherein the culture medium is chemically-defined medium or medium with added mammalian serum.
6. A three-dimensional matrix comprising an isolated cell obtained from mammalian umbilical cord tissue substantially free of blood, wherein said isolated cell is capable of self-renewal and expansion of its numbers in culture, expresses CD10, CD13, CD44, CD73, CD90, PDGFr-alpha, HLA-A, HLA-B, and HLA-C, does not express CD31, CD34, CD45, CD117, CD141, HLA-DR, HLA-DP and HLA-DQ, has increased expression, relative to a human fibroblast, mesenchymal stem cell, or iliac crest bone marrow cell, of a gene for interleukin 8, reticulon 1 and chemokine (C-X-C motif) ligand 3, and produces granulocyte chemotactic protein-2 (GCP-2), and wherein the matrix is a synthetic mat, synthetic fiber, foam, or self-assembling peptide.
7. The three-dimensional matrix of claim 6 , wherein said matrix comprises a synthetic biocompatible or bioabsorbable polymer.
8. The three-dimensional matrix of claim 7 , wherein said matrix comprises a nonwoven mat comprising a synthetic copolymer of glycolic and lactic acids, a PCL/PGA copolymer, or self-assembling peptides.
9. The three-dimensional matrix of claim 6 , wherein the isolated cell secretes MCP-1, IL-6, IL-8, GCP-2, HGF, KGF, FGF, HB-EGF, BDNF, TPO, MIP1b, RANTES, and TIMP1, and does not secrete SDF-1alpha, TGF-beta2, ANG2, PDGFbb, MIP1a, and VEGF.
10. A cryopreserved composition comprising an isolated cell obtained from mammalian umbilical cord tissue substantially free of blood, wherein said isolated cell is capable of self-renewal and expansion of its numbers, expresses CD10, CD13, CD44, CD73, CD90, PDGFr-alpha, HLA-A, HLA-B, and HLA-C, does not express CD31, CD34, CD45, CD117, CD141, HLA-DR, HLA-DP and HLA-DQ, has increased expression, relative to a human fibroblast, mesenchymal stem cell, or iliac crest bone marrow cell, of a gene for interleukin 8, reticulon 1 and chemokine (C-X-C motif) ligand 3, and produces granulocyte chemotactic protein-2 (GCP-2).
11. The cryopreserved composition of claim 10 , wherein the isolated cell secretes MCP-1, IL-6, IL-8, GCP-2, HGF, KGF, FGF, HB-EGF, BDNF, TPO, MIP1b, RANTES, and TIMP1, and does not secrete SDF-1alpha, TGF-beta2, ANG2, PDGFbb, MIP1a, and VEGF.
12. A cryopreserved composition comprising an isolated cell obtained from mammalian umbilical cord tissue substantially free of blood, wherein said cell is capable of self-renewal and expansion and wherein said cell has the characteristics of cells deposited with ATCC under Accession No. PTA-6067.
13. A cryopreserved composition comprising an isolated cell obtained from mammalian umbilical cord tissue substantially free of blood, wherein said cell is capable of self-renewal and expansion and wherein said cell has the characteristics of cells deposited with ATCC under Accession No. PTA-6068.