Immobilization support, process for producing the same, electrode, process for producing the same, electrode reaction utilizing apparatus and process for producing the same
View Patent ↗An immobilization carrier containing an electron acceptor compound is used in addition to glutaraldehyde and poly-L-lysine to immobilize an enzyme and an electron acceptor compound simultaneously to an electrode. For example, here are used diaphorase as the enzyme and 2-amino-3-carboxy-1,4-naphthoquinone (ACNQ) as the electron acceptor compound.
1. An electrode comprising:
an immobilization carrier including
glutaraldehyde, poly-L-lysine,
an electron acceptor compound wherein the electron acceptor compound has at least one amino group, and
an enzyme, and further wherein the electron acceptor compound is combined with a cross-linking agent and the electron acceptor compound functions to support cross-linking between glutaraldehyde and poly-L-lysine, and further wherein the enzyme and electron acceptor compound are immobilized simultaneously to form an enzyme-immobilized electrode, and an aldehyde group at one end of the glutaraldehyde and an amino group of the poly-L-lysine form and amide linkage and an aldehyde group at another end of the glutaraldehyde and the amino group of the enzyme simultaneously form an amide linkage to cross-link.
2. The electrode according to claim 1 , further comprising a coenzyme.
3. The electrode according to claim 2 , wherein the coenzyme has at least one amino group.