Device for the Detection of a Fluorescent Dye
The present invention relates to a device for the detection of a fluorescent dye in a sample, comprising a radiation source means with the aid of which light for exciting the fluorescent dye to be detected can be transmitted into the sample applied to a carrier, and a detecting means for detecting fluorescent light which has been emitted by the fluorescent dye to be detected. The present invention is characterized by a hollow space having an internal high-reflectance surface, a first aperture directed towards the sample, and a second aperture located opposite the detecting means.
1 . A fluorescence detection system, the system comprising:
a sample, wherein the sample comprises a plurality of fluorescent dyes;
multiple radiation sources, wherein each radiation source is configured to transmit light to excite at least one fluorescent dye in the sample; and
at least one detector configured to detect fluorescent light from at least one fluorescent dye in the sample,
wherein the detection system does not have a lens system to collect fluorescent light from the plurality of dyes, and
wherein the detection system does not have a light guide to collect fluorescent light from the plurality of dyes.
2 . The system of claim 1 , further comprising:
a high-reflectance surface, wherein the surface is configured to collect the fluorescent light from the plurality of dyes.
3 . The system of claim 2 , wherein the surface has a light-tight arrangement with the detector.
4 . The system of claim 1 , further comprising:
multiple dichroic beam dividers, wherein a single dichroic beam divider corresponds to a single radiation source.
5 . The system of claim 1 , further comprising:
a pinhole configured to fluorescence detection of a geometry of the sample.
6 . A method for detecting fluorescent dyes in a biological sample, the method comprising:
scanning excitation light of different excitation wavelengths over the carrier in two dimensions;
separating fluorescent light emitted from the carrier from the excitation wavelengths; and
passing the fluorescent light through an aperture; and
detecting the fluorescent light of multiple fluorescent dyes at different wavelengths by arranging multiple detectors with different color filters.
7 . The method of claim 6 , wherein the separating comprises providing a dichroic beam divider.