The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and method of use thereof are also provided. The nitrilases have increased activity and stability at increased pH and temperature.
1. An isolated, recombinant or synthetic polypeptide having a nitrilase activity and having at least 90% sequence identity to SEQ ID NO:190, or an enzymatically active fragment thereof.
2. The isolated, recombinant or synthetic polypeptide of claim 1 , wherein the polypeptide has at least 95% sequence identity to SEQ ID NO:190.
3. The isolated, recombinant or synthetic polypeptide of claim 2 , wherein the polypeptide has at least 98% sequence identity to SEQ ID NO:190.
4. The isolated, recombinant or synthetic polypeptide of claim 3 , wherein the polypeptide has 100%, or complete, sequence identity to SEQ ID NO:190.
5. An isolated, recombinant or synthetic polypeptide having a nitrilase activity and the amino acid sequence of SEQ ID NO:190, or an enzymatically active fragment thereof.
6. The isolated, recombinant or synthetic polypeptide of claim 1 , wherein the nitrilase activity is an enantio-selective nitrilase activity.
7. The isolated, recombinant or synthetic polypeptide of claim 1 , wherein the nitrilase activity comprises catalyzing a reaction selected from the group consisting of: