Generation of plants with improved pathogen resistance
View Patent ↗The present invention is directed to plants that display a pathogen resistance phenotype due to altered expression of a PPR2 nucleic acid. The invention is further directed to methods of generating plants with a pathogen resistance phenotype.
1. A method of producing a plant having increased pathogen resistance comprising
identifying a plant that has an allele in a nucleotide sequence that encodes a Myb polypeptide that confers Peronospora parasitica resistance (PPR2) and that results in increased pathogen resistance compared to plants lacking the allele; and
generating progeny of the identified plant,
wherein the Myb polypeptide is selected from the group consisting of: the polypeptide sequence of SEQ ID NO: 2; a polypeptide sequence having at least 95% sequence identity to SEQ ID NO: 2; and an Arabidopsis thaliana polypeptide having at least 85% sequence identity to SEQ ID NO: 2, and wherein the generated progeny inherit the allele and have the increased pathogen resistance.
2. The method of claim 1 that employs Quantitative Trait Locus (QTL) methodology.
3. The method of claim 1 that employs targeted induced local lesions in genomes (TILLING) methodology.
4. The method of claim 1 , wherein the allele alters the expression of the nucleotide sequence.
5. The method of claim 1 , wherein the allele alters the activity of the Myb polypeptide encoded by the nucleotide sequence.
6. A plant generated by the method of claim 1 .
7. A plant part obtained from the plant of claim 6 , wherein the plant part comprises the allele.
8. The plant part of claim 7 , which plant part is a seed, embryo, meristemic region, callus tissue, leaf, root, shoot, gametophyte, sporophyte, pollen, or microspore.
9. The plant of claim 6 , wherein the nucleotide sequence encodes an Arabidopsis thaliana polypeptide having at least 85% sequence identity to SEQ ID NO: 2.
10. The plant of claim 9 , wherein the nucleotide sequence encodes an Arabidopsis thaliana polypeptide having at least 90% sequence identity to SEQ ID NO: 2.
11. The plant of claim 10 , wherein the nucleotide sequence encodes an Arabidopsis thaliana polypeptide having at least 95% sequence identity to SEQ ID NO: 2.
12. The transformed plant of claim 11 , wherein the nucleotide sequence encodes SEQ ID NO: 2.
13. The transformed plant of claim 12 , wherein the nucleotide sequence comprises SEQ ID NO: 1.