Long-acting veterinary polypeptides and methods of producing and administering same
A polypeptide and polynucleotides comprising at least two carboxy-terminal peptides (CTP) of chorionic gonadotrophin attached to a non-human peptide-of-interest are disclosed. Pharmaceutical compositions comprising the non-human polypeptides and polynucleotides of the invention and methods of using both human and non-human polypeptides and polynucleotides are also disclosed.
1. A polypeptide consisting of a non-human growth hormone polypeptide, one chorionic gonadotrophin carboxy terminal peptide attached to the amino terminus of said non-human growth hormone polypeptide, and two chorionic gonadotrophin carboxy terminal peptides attached to the carboxy terminus of said non-human growth hormone polypeptide.
2. The polypeptide of claim 1 , wherein the sequence of at least a single chorionic gonadotrophin carboxy terminal peptide consists of an amino acid sequence selected from the group consisting of SEQ ID NO: 17 and SEQ ID NO: 18.
3. The polypeptide of claim 1 , wherein at least a single chorionic gonadotrophin carboxy terminal peptide is truncated.
4. A composition comprising the polypeptide of claim 1 and a pharmaceutically acceptable carrier.
5. A polypeptide consisting of a non-human growth hormone polypeptide, one chorionic gonadotrophin carboxy terminal peptide attached to the amino terminus of said non-human growth hormone polypeptide, two chorionic gonadotrophin carboxy terminal peptides attached to the carboxy terminus of said non-human growth hormone polypeptide, and a signal peptide attached to the amino terminus of said one chorionic gonadotrophin carboxy terminal peptide.
6. The polypeptide of claim 5 , wherein the sequence of said signal peptide is as set forth in SEQ ID NO: 19.
7. The polypeptide of claim 5 , wherein the sequence of at least a single chorionic gonadotropin carboxy terminal peptide consists of an amino acid sequence selected from the group consisting of SEQ ID NO: 17 and SEQ ID NO: 18.
8. The polypeptide of claim 5 , wherein at least a single chorionic gonadotrophin carboxy terminal peptide is truncated.
9. A composition comprising the polypeptide of claim 5 and a pharmaceutically acceptable carrier.
10. A method of extending the biological half life of a non-human growth hormone polypeptide, comprising the step of attaching one chorionic gonadotrophin carboxy terminal peptide to the amino terminus of said non-human growth hormone polypeptide and two chorionic gonadotrophin carboxy terminal peptides to the carboxy terminus of said non-human growth hormone polypeptide, thereby extending the biological half life of a non-human growth hormone polypeptide.
11. The method of claim 10 , wherein the sequence of at least a single chorionic gonadotrophin carboxy tei iiiinal peptide consists of an amino acid sequence selected from the group consisting of: SEQ ID NO: 17 and SEQ ID NO: 18.
12. The method of claim 10 , wherein at least a single chorionic gonadotrophin carboxy terminal peptide is truncated.
13. A method of extending the biological half life of a non-human growth hormone polypeptide, comprising the step of attaching one chorionic gonadotrophin carboxy terminal peptide to the amino terminus of said non-human growth hormone polypeptide and two chorionic gonadotrophin carboxy terminal peptides to the carboxy terminus of said non-human growth hormone polypeptide, and a signal peptide attached to the amino terminus of said one chorionic gonadotrophin carboxy terminal peptide, thereby extending the biological half life of a non-human growth hormone polypeptide.
14. The method of claim 13 , wherein the sequence of at least a single chorionic gonadotrophin carboxy terminal peptide consists of an amino acid sequence selected from the group consisting of: SEQ ID NO: 17 and SEQ ID NO: 18.
15. The method of claim 13 , wherein at least a single chorionic gonadotrophin carboxy terminal peptide is truncated.
16. The method of claim 13 , wherein the sequence of said signal peptide is as set forth in SEQ ID NO: 19.