Methods and compositions for inhibiting undesired cleaving of labels
The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. The methods of the invention include correcting one or more phenomena that are encountered during nucleotide sequencing, such as using sequencing by synthesis methods. These phenomena include, without limitation, sequence lead, sequence lag, spectral crosstalk, and noise resulting from variations in illumination and/or filter responses.
1. A method of incorporating labeled nucleotides into nucleic acid, comprising:
a) providing a plurality of nucleic acid template molecules, a polymerase, a cleaving agent, a cleaving agent scavenger comprising a disulfide bond, and a plurality of nucleotide analogues wherein each nucleotide analogue is labeled with a unique label attached through a cleavable disulfide linker and contains a removable chemical moiety capping the 3′-OH group;
b) incorporating a first nucleotide analogue with said polymerase;
c) detecting the label of the incorporated nucleotide analogue;
d) removing the chemical moiety of the incorporated nucleotide analogue capping the 3′-OH group with said cleaving agent; and
e) incorporating a second nucleotide analogue in the presence of said cleaving agent scavenger.
2. The method of claim 1 , wherein said removable chemical moiety comprises a disulfide bond.
3. The method of claim 1 , wherein said removable chemical moiety comprises an azido group.
4. The method of claim 1 , wherein said removable chemical moiety comprises an azidomethyl ether.
5. The method of claim 1 , wherein said cleaving agent is a phosphine.
6. The method of claim 5 , wherein said phosphine is Tris(2-carboxy-ethyl)phosphine.
7. The method of claim 1 , wherein said cleaving agent scavenger does not contain a nucleic acid base.
8. The method of claim 1 , wherein said scavenger is cystamine.
9. The method of claim 1 , further comprising, prior to step b) hybridizing a primer to said plurality of nucleic acid template molecules, such that said first nucleotide analogue is incorporated into said primer at step b).
10. The method of claim 1 , wherein said nucleic acid template molecules comprise a self-priming hairpin, such that said first nucleotide analogue is incorporated into said template molecules at step b).
11. A method of incorporating labeled nucleotides into nucleic acid, comprising:
a) providing a plurality of nucleic acid template molecules, a polymerase, a cleaving agent, a cleaving agent scavenger comprising an azido group, and a plurality of nucleotide analogues selected from the group consisting of cytosine, thymine, deaza-adenine and deaza-guanine, wherein each nucleotide analogue comprises a unique label attached through a cleavable linker to a 5-position of cytosine or thymine or to a 7-position of deaza-adenine or deaza-guanine, and wherein each nucleotide analogue contains a removable chemical moiety capping the 3′-OH group;
b) incorporating a first nucleotide analogue with said polymerase;
c) detecting the label of the incorporated nucleotide analogue;
d) removing the chemical moiety of the incorporated nucleotide analogue capping the 3′-OH group and cleaving the cleavable linker with said cleaving agent; and
e) incorporating a second nucleotide analogue in the presence of said cleaving agent scavenger.
12. The method of claim 11 , further comprising, prior to step b) hybridizing a primer to said plurality of nucleic acid template molecules, such that said first nucleotide analogue is incorporated into said primer at step b).
13. The method of claim 11 , wherein said nucleic acid template molecules comprise a self-priming hairpin, such that said first nucleotide analogue is incorporated into said template molecules at step b).
14. The method of claim 11 , wherein said cleavable linker comprises a disulfide bond.
15. The method of claim 11 , wherein said removable chemical moiety comprises an azido group and said cleavable linker comprises a disulfide bond.
16. The method of claim 11 , wherein said removable chemical moiety comprises an azidomethyl ether.
17. The method of claim 11 , wherein said cleaving agent is a phosphine.
18. The method of claim 17 , wherein said phosphine is (Tris-carboxyethyl)phosphine.
19. The method of claim 11 , wherein said cleaving agent scavenger does not contain a nucleic acid base.
20. The method of claim 11 , wherein said scavenger comprises an azidomethyl group.
21. The method of claim 11 , wherein said scavenger comprises an azidoethyl ether group.
22. The method of claim 11 , wherein said scavenger is 11-Azido-3,6,9-trioxaundecan-1-amine.
23. A method of incorporating labeled nucleotides into nucleic acid, comprising:
a) providing a plurality of nucleic acid template molecules, a polymerase, a cleaving agent, cystamine, and a plurality of nucleotide analogues wherein each nucleotide analogue is labeled with a unique label, said label attached by a cleavable linker comprising a disulfide bond;
b) incorporating a first nucleotide analogue with said polymerase;
c) detecting the label of the incorporated nucleotide analogue;
d) removing the label of the incorporated nucleotide analogue by cleaving the cleavable linker with said cleaving agent; and
e) incorporating a second nucleotide analogue in the presence of said cystamine.
24. The method of claim 23 , further comprising, prior to step b) hybridizing a primer to said plurality of nucleic acid template molecules, such that said first nucleotide analogue is incorporated into said primer at step b) and said second nucleotide analogue is incorporated into said primer at step e).
25. The method of claim 23 , wherein said nucleic acid template molecules comprise a self-priming hairpin, such that said first nucleotide analogue is incorporated into said template molecules at step b) and said second nucleotide analogue is incorporated into said template molecules at step e).
26. The method of claim 23 , wherein said cleaving agent is a phosphine.
27. The method of claim 26 , wherein said phosphine is (Tris-carboxyethyl)phosphine.
28. The method of claim 23 , wherein said cleaving agent scavenger does not contain a nucleic acid base.
29. A method of incorporating labeled nucleotides into nucleic acid, comprising:
a) providing a plurality of nucleic acid template molecules, a polymerase, a cleaving agent, 11-Azido-3,6,9-trioxaundecan-1-amine, and a plurality of nucleotide analogues wherein each nucleotide analogue is labeled with a unique label and contains a removable chemical moiety capping the 3′-OH group;
b) incorporating a first nucleotide analogue with said polymerase;
c) detecting the label of the incorporated nucleotide analogue;
d) removing the chemical moiety of the incorporated nucleotide analogue capping the 3′-OH group with said cleaving agent; and
e) incorporating a second nucleotide analogue in the presence of said 11-Azido-3,6,9-trioxaundecan-1-amine.
30. The method of claim 29 , wherein said removable chemical moiety comprises an azido group.
31. The method of claim 29 , wherein said removable chemical moiety comprises an azidomethyl ether.