WHOLE GENOME EXPRESSION ANALYSIS SYSTEM
A method for simultaneously determining a genetic expression profile for an individual member of a species relative to an entire standard genome for the species. The method can comprise distributing a liquid sample into an array of reaction chambers of a substrate. The array can comprise a primer set and a probe for each polynucleotide target along the entire standard genome. The liquid sample can comprise substantially all genetic material of the member. Each of the reaction chambers can comprise the primer set and the probe for at least one of the polynucleotide targets and a polymerase. The method can further comprise amplifying the liquid sample in the array, detecting a signal emitted by at least one of the probes, and identifying the genetic expression profile in response to the signal.
1 . A method for determining a genetic expression profile for an individual member of a species, said method comprising:
distributing a liquid sample into an array of reaction chambers of a substrate, said array having a primer set and a probe for each of a plurality of expressed polynucleotide targets, said liquid sample having substantially all expressed genetic material of said member, each of said reaction chambers having a volume of less than or equal to 1 microliter and having said primer set and said probe for at least one of said plurality of expressed polynucleotide targets and a polymerase;
amplifying said liquid sample in said array;
detecting a signal emitted by at least one of said probes; and
identifying said genetic expression profile in response to said signal.
2 . The method according to claim 1 wherein said amplifying said liquid sample in said array includes amplifying said liquid sample using PCR.
3 . The method according to claim 2 wherein said amplifying said liquid sample using PCR includes amplifying said liquid sample using real time PCR.
4 . The method according to claim 2 wherein said amplifying said liquid sample using PCR includes amplifying said liquid sample using multiplex PCR.
5 . The method according to claim 1 , further comprising:
forcing said liquid sample into said reaction chambers after said distributing said liquid sample into said array of reaction chambers.
6 . The method according to claim 5 wherein said forcing said liquid sample into said reaction chambers includes forcing said liquid sample into said reaction chambers using centrifugal force.
7 . A method for determining a genetic expression profile for an individual member of a species, said method comprising:
distributing a liquid sample into an array of reaction chambers of a substrate by forcing said liquid sample into said reaction chambers using pneumatic pressure, said array having a primer set and a probe for each of a plurality of expressed polynucleotide targets, said liquid sample having substantially all expressed genetic material of said member, each of said reaction chambers having said primer set and said probe for at least one of said plurality of expressed polynucleotide targets and a polymerase;
amplifying said liquid sample in said array;
detecting a signal emitted by at least one of said probes; and
identifying said genetic expression profile in response to said signal.
8 . The method according to claim 1 , further comprising:
preamplifying said liquid sample before said distributing said liquid sample into said array of reaction chambers.
9 . The method according to claim 1 , further comprising:
sealing each of said reaction chambers with a sealing cover prior to amplifying said liquid sample in said array.
10 . The method according to claim 1 wherein said distributing said liquid sample comprises:
flooding a surface of said substrate with said liquid sample.
11 . The method according to claim 1 , further comprising:
distributing amplification reactants to said reaction chambers prior to amplifying said liquid sample in said array.
12 . A method for determining a genetic expression profile for an individual member of a species, said method comprising:
distributing a liquid sample into an array of reaction chambers of a substrate by spraying a surface of said substrate with said liquid sample, said array having a primer set and a probe for each of a plurality of expressed polynucleotide targets, said liquid sample having substantially all expressed genetic material of said member, each of said reaction chambers having said primer set and said probe for at least one of said plurality of expressed polynucleotide targets and a polymerase;
amplifying said liquid sample in said array;
detecting a signal emitted by at least one of said probes; and
identifying said genetic expression profile in response to said signal.