Immobilized biologically active entities having high biological activity following mechanical manipulation
View Patent ↗The present invention is directed to immobilized biologically active entities that retain significant biological activity following mechanical manipulation of a substrate material to which the entities are immobilized.
1. A medical device comprising:
a medical device that is capable of being compacted and expanded;
a polymeric substrate material, wherein said polymeric substrate material is fluoropolymeric in composition;
a polymeric covering material attached to at least a portion of a surface of said substrate material;
a first plurality of heparin molecules having anti-thrombin Ill binding activity end point attached to at least a portion of said polymeric covering material; and
a composition comprising a second plurality of heparin molecules non-covalently combined with said first plurality of heparin molecules,
wherein said first plurality of heparin molecules have an anti-thrombin Ill binding activity of at least 100 picomoles anti-thrombin III per square centimeter (pmol/cm 2 ) substrate material when measured following compaction of said medical device to 50% of said medical device's original size and expansion of said medical device.
2. The medical device of claim 1 wherein said fluoropolymeric material is polytetrafluoroethylene.
3. The medical device of claim 1 , further comprising an antiproliferative agent.
4. The medical device of claim 3 wherein said antiproliferative agent is dexamethasone.