All-in-one means of separating blood components
View Patent ↗A separation device including a first buoy, a second buoy, a first valve, and a second valve. The first buoy is mounted to a buoy guide post and slidably mounted within a separation chamber. The second buoy is slidably mounted to the guide post and movable between a first position and a second position. The second buoy closes the first valve and opens the second valve when in the first position. The second buoy opens the first valve and closes the second valve when in the second position. The second buoy has a density such that after spinning the device for a suitable period of time a first component of the composition is isolated between the first buoy and the second buoy and a second component of the composition is isolated between the second buoy and the end of the separation chamber that is opposite to a port.
1. A method for separating whole blood into different components comprising:
drawing the whole blood directly from a patient into a separation chamber through a port of the separation chamber, the separation chamber having a first buoy fixedly mounted to a buoy guide post and a second buoy slidably mounted to the buoy guide post, the buoy guide post having a first valve and a second valve, said first valve is closed and said second valve is open when said second buoy is in a first position, the whole blood is drawn into the separation chamber through the first buoy, the second buoy, and the second valve into an area between the second buoy and a plunger slidably mounted in the separation chamber;
rotating the chamber in a centrifuge for a sufficient period of time such that the second buoy moves to a second position in which the second buoy is spaced apart from the first buoy to close the second valve and open the first valve and the components of the whole blood separate according to density such that red blood cells are between the port and the first buoy, platelet rich plasma is between the first buoy an the second buoy, and platelet poor plasma is between the second buoy and the plunger; and
depressing the plunger to a first distance within the separation chamber to move the second buoy from the second position to the first position to force the platelet rich plasma through the first valve and out of the separation chamber through the port, to close the first valve, and to open the second valve.
2. The method of claim 1 , wherein the whole blood is drawn into the separation chamber through the port using a needle tip connected to the port with an extension nozzle.
3. The method of claim 1 , further comprising:
applying the platelet rich plasma directly to an area of interest through an applicator attached to the port;
depressing the plunger to a second distance within the separation chamber that is greater than the first distance to force the platelet poor plasma through the second valve and out of the separation chamber through the port; and
applying the platelet poor plasma directly to an area of interest through an applicator attached to the port.
4. The method of claim 3 , wherein the platelet poor plasma is applied through an applicator attached directly to the port.
5. The method of claim 3 , further comprising applying the platelet rich plasma and the platelet poor plasma directly to a wound site through a spray tip attached to the port.
6. The method of claim 1 , further comprising drawing the whole blood into a syringe that includes the separation chamber.
7. The method of claim 1 , further comprising rotating the separation chamber such that both the first buoy and the second buoy move along a longitudinal axis of the separation chamber.
8. The method of claim 1 , further comprising detaching a plunger handle from a plunger body of the plunger prior to rotating the separation chamber in the centrifuge and reattaching the plunger handle to the plunger body after the chamber is rotated in the centrifuge.
9. The method of claim 1 , further comprising restricting movement of the plunger prior to rotating the chamber.
10. A method for separating bone marrow aspirate into different components comprising:
drawing the bone marrow aspirate directly from a patient into a separation chamber through a port of the separation chamber, the separation chamber having a first buoy fixedly mounted to a buoy guide post and a second buoy slidably mounted to the guide post, the buoy guide post having a first valve and a second valve, said first valve is closed and said second valve is open when said second buoy is in a first position, the bone marrow aspirate is drawn into the separation chamber through the first buoy, the second buoy, and the second valve into an area between the second buoy and a plunger slidably mounted in the separation chamber;
rotating the chamber in a centrifuge for a sufficient period of time such that the second buoy moves to a second position in which the second buoy is spaced apart from the first buoy to close the second valve and open the first valve and separate the components of the bone marrow aspirate according to density such that red blood cells are between the port and the first buoy, multipotent cells are between the first buoy an the second buoy, and bone marrow plasma is between the second buoy and the plunger; and
depressing the plunger to a first distance within the separation chamber to move the second buoy from the second position to the first position to force the multipotent cells through the first valve and out of the separation chamber through the port, to close the first valve, and to open the second valve.
11. The method of claim 10 , further comprising:
applying the multipotent cells directly to an area of interest through an applicator attached to the port:
depressing the plunger to a second distance within the separation chamber that is greater than the first distance to force the bone marrow plasma through the second valve and out of the separation chamber through the port; and
applying the bone marrow plasma directly to an area of interest through an applicator attached to the port.
12. A method for separating whole blood into different components comprising:
drawing the whole blood directly from a patient into a separation chamber through a port, the separation chamber having a first buoy slidably mounted in the separation chamber and a second buoy slidably mounted in the separation chamber, the whole blood is drawn into the separation chamber through the first buoy and through the second buoy into an area between the second buoy and a plunger slidably mounted in the separation chamber;
rotating the chamber in a centrifuge for a sufficient period of time such that the second buoy moves from a first position to a second position in which the second buoy is spaced apart from the first buoy and the components of the whole blood separate according to density such that red blood cells are between the port and the first buoy, platelet rich plasma is between the first buoy and the second buoy, and platelet poor plasma is between the second buoy and the plunger; and
depressing the plunger to a first distance within the separation chamber to move the second buoy from the second position to the first position to force the platelet rich plasma out of the separation chamber through the port.
13. The method of claim 12 , further comprising drawing the whole blood into the separation chamber through a needle tip attached to the port with an extension nozzle.
14. The method of claim 12 , further comprising:
applying the platelet rich plasma directly to an area of interest through an applicator attached to the port;
further depressing the plunger to a second distance within the separation chamber that is greater than the first distance to force the platelet poor plasma out of the separation chamber through the port; and
applying the platelet poor plasma directly to an area of interest through the applicator attached to the port.
15. The method of claim 14 , wherein the platelet poor plasma is applied to the area of interest with the applicator attached directly to the port.