Δ-15 desaturase genes suitable for increasing levels of omega-3 fatty acids
The present invention relates to fungal Δ-15 fatty acid desaturases that are able to catalyze the conversion of linoleic acid (18:2, LA) to alpha-linolenic acid (18:3, ALA). Nucleic acid sequences encoding the desaturases, nucleic acid sequences which hybridize thereto, DNA constructs comprising the desaturase genes, and recombinant host plants and microorganisms expressing increased levels of the desaturases are described. Methods of increasing production of specific omega-3 and omega-6 fatty acids by over-expression of the Δ-15 fatty acid desaturases are also described herein.
1. Transgenic seeds obtained from an oilseed plant, plant cell, plant tissue or plant part, comprising in its genome a recombinant construct comprising an isolated nucleic acid fragment encoding a fungal Δ15 desaturase enzyme, selected from the group consisting of:
a) an isolated nucleic acid fragment encoding the amino acid sequence as set forth in SEQ ID NO:2;
b) an isolated nucleic acid fragment that hybridizes over the full length of the isolated nucleic acid fragment of (a) under the following hybridization conditions: 0.1×SSC, 0.1% SDS, 65 C and washed with 2×SSC, 0.1% SDA followed by 0.1×SSC, 0.1% SDA; and
an isolated nucleic acid fragment that is complementary over the full length of (a) or (b), wherein said seeds comprise the nucleic acid fragment of a) or b).