Cartridge and Method for Sample Analysis
The invention provides a cartridge containing an addressable array for detecting the presence of one or more target analytes in a fluid sample. The cartridge comprises (i) a housing defining a sample inlet, an optical cell, an outlet, a first conduit in fluidic communication with the sample inlet and the optical cell, and a second conduit in fluidic communication with the outlet and the optical cell, (ii) an addressable array disposed within the optical cell; and (iii) a reagent dried upon a fluid contacting surface of at least one of the sample inlet and the first conduit, such that, when a fluid sample is applied to the fluid inlet, the fluid sample mobilizes and transports the reagent to the optical cell. The invention also provides a method of detecting one or more analytes in a fluid sample of interest.
1 . A cartridge for detecting presence of a target analyte in a fluid sample, the cartridge comprising:
a housing defining a sample inlet, an optical cell, an outlet, a first conduit in fluidic communication with the sample inlet and the optical cell, and a second conduit in fluidic communication with the outlet and the optical cell;
an addressable array disposed within the optical cell; and
a reagent dried upon a fluid contacting surface of at least one of the sample inlet and the first conduit, such that, when a fluid sample is applied to the fluid inlet, the fluid sample mobilizes and transports the reagent to the optical cell.
2 . The cartridge of claim 1 , wherein the dried reagent comprises a detectable label.
3 . The cartridge of claim 2 , wherein the dried reagent comprises a chemical moiety capable of chemically coupling the detectable label to the target analyte.
4 . The cartridge of claim 1 , wherein the dried reagent comprises a binder for the analyte.
5 . The cartridge of claim 4 , wherein the binder for the analyte is associated with a detectable label.
6 . The cartridge of any one of claims 1 , wherein the second conduit defines a first volume.
7 . The cartridge of claim 6 , wherein the sample inlet defines a sample well.
8 . The cartridge of claim 7 , wherein the sample well defines a second volume, wherein the first volume is greater than the second volume.
9 . The cartridge of claim 6 , wherein the optical cell defines a third volume, wherein the first volume is greater than the third volume.
10 . The cartridge of any one of claims 1 , wherein the cartridge further comprises a second reagent dried on a fluid contacting surface of the inlet conduit at a location downstream of the first reagent, such that fluid sample contacts the first reagent before contacting the second reagent.
11 . The cartridge of claim 10 , wherein the second reagent is capable of binding the first reagent.
12 . The cartridge of any one of claims 1 , wherein the outlet is adapted to cooperate with a pump external to the cartridge.
13 . The cartridge of any one of claims 1 , wherein the array comprises a plurality of spaced apart regions.
14 . The cartridge of claim 13 , wherein each region comprises an immobilized binder for an analyte.
15 . The cartridge of claim 14 , wherein each region comprises plurality of immobilized binders, wherein each binder binds a preselected analyte.
16 . The cartridge of claim 14 , wherein a first binder immobilized in a first region binds a first preselected analyte and a second binder immobilized in a second region binds a second, different preselected analyte.
17 . The cartridge of any one of claims 13 , wherein the array comprises at least 5 regions, each of which is capable of binding a separate analyte.
18 . The cartridge of claim 17 , wherein the array comprises at least 10 regions, each of which is capable of binding a separate analyte.
19 . The cartridge of claim 18 , wherein the array comprises at least 50 regions, each of which is capable of binding a separate analyte.
20 . The cartridge of any one of claims 1 , wherein the array is disposed upon a base of the optical cell.
21 . The cartridge of claim 20 , wherein the array is disposed directly on the base of the optical cell.
22 . The cartridge of claim 21 , wherein the array is disposed upon a solid support separate from the base of the optical cell.
23 . The cartridge of any one of claims 1 , wherein at least a portion of the optical cell is substantially transparent.
24 . The cartridge of any one of claims 1 , wherein the sample inlet is defined at least in part by an upper portion of the cartridge.
25 . A method of detecting presence of a target analyte in a fluid sample, the method comprising the steps of:
(a) applying a fluid sample to the sample inlet of the cartridge of any one of claims 1 ; and
(b) detecting the presence of a signal produced at the addressable array, wherein the presence of signal is indicative of the presence of a preselected analyte in the sample.
26 . The method of claim 25 , wherein a plurality of signals are detected at different locations of the array, and wherein each signal is indicative of the presence of different analytes in the fluid sample.