Inactivated nodavirus vaccine
View Patent ↗A vaccine against nodavirus infection in fish can be produced by inactivating the virus using an aziridine compound. This vaccine can be used to prevent Viral Nervous Necrosis (VNN) in a variety of fish species.
1. An inactivated piscine viral nervous necrosis virus (NNV) strain Mt/01/Sba deposited with the ECACC under Accession No. 03060401, wherein the inactivated piscine viral nervous necrosis virus when administered to fish, is capable of providing a relative percent survival (RPS) of at least 50% after challenge with a non-inactivated NNV strain of Mt/01/Sba, and wherein the inactivated piscine viral nervous necrosis virus is inactivated with binary ethyleneimine.
2. A vaccine composition comprising:
an inactivated piscine viral nervous necrosis virus (NNV) strain Mt/01/Sba deposited with ECACC under Accession No. 03060401; and
a pharmaceutically acceptable carrier,
wherein the inactivated piscine viral nervous necrosis virus is inactivated with binary ethyleneimine.
3. The vaccine composition of claim 2 , wherein the vaccine is capable of providing a relative percent survival (RPS) of at least 77% in a fish population vaccinated with the vaccine subsequent to challenge with a non-inactivated NNV strain of Mt/01/Sba.
4. The vaccine composition of claim 3 , wherein the RPS is at least 91.55%.
5. The vaccine composition of claim 2 , further comprising about 10 2 to about 10 9 TCID 50 of the inactivated nodavirus per unit dose.
6. The inactivated piscine viral nervous necrosis virus of claim 1 , wherein when administered to fish, is capable of providing a relative percent survival (RPS) of at least 77% after challenge with a non-inactivated NNV strain of Mt/01/Sba.
7. The inactivated piscine viral nervous necrosis virus of claim 1 , wherein when administered to fish, is capable of providing a relative percent survival (RPS) of at least 91% after challenge with a non-inactivated NNV strain of Mt/01/Sba.
8. The vaccine composition of claim 2 , further comprising an adjuvant.