Method for multiplexed capillary electrophoresis signal cross-talk correction
View Patent ↗The present invention provides a simple method to correct cross-talk, after the data have been generated. Adjacent signals are simply subtracted from the original observed signal with a repeating process. The data processing is stopped when a predefined condition is met. By this technique, cross-talk can be reduced from >5% to less than 0.1%. And as an additional advantage, this method provides a way to correct the cross-talk without the need to know which peaks are caused by the adjacent capillary signal.
1. A method of correcting multiplexed capillary electrophoresis (CE) electropherograms for cross-talk, comprising:
preparing an observed signal electropherogram for a selected capillary of a multi-capillary electrophoresis (CE) system;
preparing an observed signal (CE) electropherogram for the adjacent capillaries on each side of the selected capillary;
selecting an arbitrary percentage that is less than 100% for designation as A % that is multiplied with the observed selected signal, the product being subtracted out from the observed selected signal for each adjacent capillary;
repeating the A % multiplication with reduced A % value, and subtraction out process as long as it gives a negative value, and/or until the subtracted value is larger than an arbitrary preset B value:
preparing a corrected multiplexed capillary electrophoresis electropherogram.
2. The process of claim 1 wherein the A % value is from 5% to 20% of the adjacent capillary signal.
3. The process of claim 2 wherein the B value is between −0.1% and −0.5% of the adjacent capillary signal.