Alteration of oil traits in plants
The preparation and use of nucleic acid fragments useful in altering the oil phenotype in plants are disclosed. Chimeric constructs incorporating such nucleic acid fragments and suitable regulatory sequences can be used to create transgenic plants having altered lipid profiles. Methods for altering the oil phenotype in plants using such nucleic acid fragments also are disclosed.
1. A chimeric construct comprising an isolated nucleic acid operably linked to at least one regulatory sequence, wherein the isolated nucleic acid comprises a nucleic acid sequence encoding a polypeptide having an amino acid sequence of at least 95% sequence identity based on the Clustal method of alignment when compared to SEQ ID NO: 481, wherein said polypeptide is capable of altering oil phenotype in a plant, and wherein the isolated nucleic acid and the at least one regulatory sequence are not found together in nature.
2. A plant comprising in its genome the chimeric construct of claim 1 .
3. The plant of claim 2 wherein said plant is selected from the group consisting of corn, soybean, wheat, rice, canola, Brassica , sorghum, sunflower, and coconut.
4. A method for altering oil phenotype in a plant which comprises:
(a) transforming a plant with the chimeric construct of claim 1 ;
(b) growing the transformed plant under conditions suitable for expression of the chimeric construct; and
(c) selecting those transformed plants whose oil phenotype has been altered compared to the oil phenotype of an untransformed plant.
5. The method of claim 4 wherein the plant is selected from the group consisting of corn, soybean, wheat, rice, canola, Brassica , sorghum, sunflower, and coconut.
6. The chimeric construct of claim 1 , wherein the polypeptide has an amino acid sequence of at least 98% sequence identity based on the Clustal method of alignment when compared to SEQ ID NO: 481.
7. The chimeric construct of claim 1 , wherein the polypeptide has an amino acid sequence of at least 99% sequence identity based on the Clustal method of alignment when compared to SEQ ID NO: 481.
8. The chimeric construct of claim 1 , wherein the polypeptide comprises the amino acid sequence of SEQ ID NO: 481.
9. The chimeric construct of claim 1 , wherein the nucleic acid comprises the nucleic acid sequence of SEQ ID NO: 480.
10. A seed comprising in its genome the chimeric construct of claim 1 .
11. The seed of claim 10 , wherein said seed is selected from the group consisting of corn, soybean, wheat, rice, canola, Brassica , sorghum, sunflower, and coconut.