Preparation of a therapeutic composition
View Patent ↗Product R, a novel therapeutic composition for treating viral infections and stimulating the immune system comprises a unique peptide having 31 amino acids and another unique peptide having 21 amino acids and connected with an oligo-nucleotide through a diphosphodiester or diphosphodithioate ester linkage. The composition has a light absorption spectrum with typical absorption ratios of 1.998 at 260 mn/280 nm and 1.359 at 260 nm/230 nm.
1. A method for producing a peptide-nucleic acid product comprising the steps of:
a) suspending about 35.0 grams of casein, about 17.1 grams of beef peptone, about 22.0 grams of ribonucleic acid (RNA), and about 3.25 grams of bovine serum albumin in about 2.5 liters of water;
b) adding about 11.75 ml of hydrochloric acid to the mixture from step a;
c) autoclaving the product from step b at about 9 lbs pressure and 200-230° F. until the RNA is completely digested;
d) cooling the product from step c to about 3-8° C.;
e) sequentially filtering the product from step d through a 2 micron filter, a 0.45 micron filter and a 0.2 micron filter;
f) diluting the product from step e with water to yield a final volume of about 5 liters;
g) adjusting the pH of the product from step f to a range of about 7.3 to 7.6;
h) filtering the product from step g through a second 0.2 micron filter; and
i) autoclaving the product from step h at 240° F. and 20-30 pounds pressure for about 30 minutes.
2. The method of claim 1 , wherein step e is repeated.
3. A product produced by the method of claim 1 .
4. A product produced by the method of claim 2 .
5. A pharmaceutical composition comprising a) the product of claim 3 ; and b) a pharmaceutically acceptable carrier.