TISSUE ENGINEERED BLOOD VESSELS
Compositions and methods of using tissue engineered blood vessels to repair and regenerate blood vessels of patients with vascular disease are disclosed.
1 . A tubular construct for a tissue engineered blood vessel comprising a scaffold comprising poly(p-dioxanone) and one or more or of cells, cell sheets, cell lysate and minced tissue.
2 . The tubular construct of claim 1 wherein the scaffold further comprises collagen.
3 . The tubular construct of claim 1 wherein the cells are selected from the group consisting of endothelial cells, smooth muscle cells, fibrobasts, human umbilical cord derived cells and combinations thereof.
4 . A method of making a tissue engineered blood vessel comprising the steps of:
a. Providing a tubular shaped scaffold comprising poly(p-dioxanone);
b. Seeding said scaffold with one or more cells selected from the group consisting of endothelial cells, smooth muscle cells, fibroblasts and human umbilical cord-derived cells; and
c. Culturing the cell seeded scaffold in a bioreactor.
5 . A method of making a tissue engineered blood vessel comprising the steps of:
a. Providing a tubular shaped scaffold comprising poly(p-dioxanone);
b. Wrapping said scaffold with a human umbilical cord-derived cell sheet; and
c. Culturing the cell sheet wrapped scaffold in a bioreactor.
6 . A method of making a tissue engineered blood vessel comprising the steps of:
a. Providing a tubular shaped scaffold comprising poly(p-dioxanone);
b. Applying said scaffold with minced muscle tissue and minced endothelial tissue; and
c. Culturing the minced tissue scaffold in a bioreactor.
7 . A method of making a tissue engineered blood vessel comprising the steps of:
a. Providing a tubular shaped scaffold comprising poly(p-dioxanone);
b. Applying said scaffold with cell lysate; and then
c. Lyophilizing the scaffold.
8 . The method of claim 7 further comprising the steps of:
a. applying one or more of cells and minced tissue to the cell lysate scaffold; and
b. culturing the cell lysate scaffold having one or more of cells and minced tissue in a bioreactor.
9 . A method of making a tissue engineered blood vessel comprising the steps of:
a. Providing a scaffold comprising poly(p-dioxanone);
b. Seeding said scaffold sheet with cells;
c. Rolling said seeded sheet into a tube; and
d. Culturing the tube in a bioreactor.