Method for production of L-glutamine
View Patent ↗According to the present invention, it is possible to provide a microorganism belonging to the genus Corynebacterium , wherein the activity of (1) a protein having the amino acid sequence shown by any one of SEQ ID NO:1 to 3, or (2) a protein having a homology of 80% or more to the amino acid sequence shown by any one of SEQ ID NO:1 to 3, and having substantially the same activity as the activity of a protein having the amino acid sequence shown by any one of SEQ ID NO:1 to 3, has been reduced or lost, and wherein the activity of (3) a protein having the amino acid sequence shown by SEQ ID NO:4, or (4) a protein having a homology of 80% or more to the amino acid sequence shown by SEQ ID NO:4, and having substantially the same activity as the activity of a protein having the amino acid sequence shown by SEQ ID NO:4, has been reduced or lost, and a process for producing L-glutamine using the microorganism and the like.
1. A mutant microorganism belonging to the genus Corynebacterium , which has been formed by mutating a parental microorganism belonging to the genus Corynebacterium,
wherein the mutant microorganism produces L-glutamine more efficiently than the parental microorganism,
wherein the parental microorganism exhibits
(a) the activity of (1) a protein having the amino acid sequence shown by any one of SEQ ID NO: 1 to 3, or (2) a protein having a homology of 95% or more to the amino acid sequence shown by any one of SEQ ID NO: 1 to 3, and having substantially the same activity as the activity of a protein having the amino acid sequence shown by any one of SEQ ID NO: 1 to 3, and
(b) the activity of (3) a protein having the amino acid sequence shown by SEQ ID NO: 4, or (4) a protein having a homology of 95% or more to the amino acid sequence shown by SEQ ID NO: 4, and having substantially the same activity as the activity of a protein having the amino acid sequence shown by SEQ ID NO: 4,
wherein a nucleotide deletion, substitution, or addition to the nucleotide sequence of an endogenous gene of the parental microorganism results in the reduction or loss of the protein activity (a), and
wherein a nucleotide deletion, substitution, or addition to the nucleotide sequence of an endogenous gene of the parental microorganism results in the reduction or loss of the protein activity (b).
2. The microorganism of claim 1 , wherein the microorganism belonging to the genus Corynebacterium is Corynebacterium glutamicum.
3. A process for producing L-glutamine, comprising
culturing the microorganism of claim 1 or a mutant microorganism belonging to the genus Corynebacterium wherein a parental microorganism belonging to the genus Corynebacterium has been mutated so that the activity of one or more proteins selected from
(1) a protein having the amino acid sequence shown by SEQ ID NO: 1,
(2) a protein having the amino acid sequence shown by SEQ ID NO: 2,
(3) a protein having the amino acid sequence shown by SEQ ID NO: 3,
(4) a protein having the amino acid sequence shown by SEQ ID NO: 4, and
(5) a protein having a homology of 95% or more to the amino acid sequence shown by any one of SEQ ID NO: 1 to 4, and having substantially the same activity as the activity of any one of the proteins (1) to (4),
has been reduced or lost relative to the activity of the corresponding one or more proteins in the parental microorganism, in a medium to produce and accumulate L-glutamine in the culture, and
recovering L-glutamine from the culture,
wherein the reduction or loss of the activity of each of the one or more proteins is effected by a nucleotide deletion, substitution, or addition to the nucleotide sequence of an endogenous gene encoding each of the one or more proteins, and
wherein the mutant microorganism produces L-glutamine more efficiently than the parental microorganism.
4. The process for producing L-glutamine of claim 3 , wherein the microorganism belonging to the genus Corynebacterium is Corynebacterium glutamicum.