Medium supplement for virus production
View Patent ↗The use of macrolide polyene antibiotics or derivatives or analogues thereof as culture supplement for the propagation of virus is described. Further pharmaceutical compositions comprising a virus and a macrolide polyene antibiotic or a derivative or analogue thereof and methods for using of macrolide polyene antibiotics for transfection and infection of cells as well as the use of macrolide polyene antibiotics for the isolation of virus from clinical samples are disclosed.
1. A method of propagating influenza virus, comprising the step of adding amphotericin B to a cell culture comprising VERO cells and the influenza virus.
2. The method of claim 1 , wherein the amphotericin B is added to the cell culture at a concentration of between 0.5 ng/ml and 5 microg/ml.
3. The method of claim 2 , wherein the amphotericin B is at a concentration of between 0.5 ng/ml and 2.5 μg/ml.
4. The method of claim 3 , wherein the amphotericin B is at a concentration of between 10 ng/ml and 900 ng/ml.
5. The method of claim 4 , wherein the amphotericin B is at a concentration of between 100 ng/ml and 500 ng/ml.
6. The method of claim 5 , wherein the amphotericin B is at a concentration of between 200 ng/ml and 400 ng/ml.
7. The method of claim 1 , wherein the influenza virus is selected from the group consisting of influenza A virus, influenza B virus, and influenza C virus.
8. The method of claim 1 , wherein the influenza virus contains a modification within the NS1 or PB1 gene.
9. The method of claim 8 , wherein the modification is a deletion, substitution or insertion of at least one nucleic acid.
10. The method of claim 1 wherein virus growth in the cell culture is increased compared to virus growth in the absence of amphotericin B by an amount of at least 0.1 log 10, preferably at least 0.5 log 10, more preferably at least 1 log 10, more preferably at least 2 log 10, and even more preferably at least 2.5 log 10.
11. A method of transfecting Vero cells, comprising the step of adding amphotericin B to a cell culture comprising Vero cells before, simultaneously with or shortly after transfecting the Vero cells with expression plasmids containing viral RNA segments of influenza virus.
12. A method for the infection of cells for influenza virus cultivation using amphotericin B, comprising the following steps:
a) infecting cells with at least one infectious influenza virus particle;
b) adding at least amphotericin B to an inoculum or cultivation media together with trypsin to form a cell culture mixture;
c) incubating the cells with the cell culture mixture; followed by
d) harvesting of influenza viruses.
13. The method of claim 12 , further comprising the step of purifying the harvested virus.
14. The method of claim 12 , further comprising the step of characterizing the harvested virus.