Turkey herpesvirus vectored recombinant containing avian influenza genes
The present invention provides a recombinant turkey herpesvirus modified by the presence of the cDNA encoding the hemagglutinin protein of avian influenza virus under a promoter. A poultry vaccine comprising the recombinant turkey herpesvirus described in the present invention can induce serological responses that may be easily detected by the hemagglutination inhibition assay but not by commercially available diagnostic ELISA kits; thus enabling easy differentiation between vaccination and field infection.
1. A recombinant turkey herpesvirus comprising a hemagglutinin gene of avian influenza virus and the cytomegalovirus immediate early promoter, wherein said hemagglutinin gene is under control of said promoter,
wherein said avianinfluenza virus is H5 subtype.
2. A recombinant turkey herpesvirus comprising a hemagglutinin gene of avian influenza virus and the cytomegalovirus immediate early promoter, wherein said hemagglutinin gene is under control of said promoter,
wherein said avian influenza virus is A/Turkey/Wisconsin/68 (H5N9) strain.
3. A recombinant turkey herpesvirus comprising a hemagglutinin gene of avian influenza virus and the cytomegalovirus immediate early promoter, wherein said hemagglutinin gene is under control of said promoter,
wherein the nucleotide sequence of said hemagglutinin gene is shown in SEQ ID NO: 1.