Cell culture method
In a cell culture in a sealed cell culture container, gradual deterioration of culture environment is prevented. A cell culture method in which cell culture is conducted by adding a fresh culture medium to a culture container in which cells and a culture medium are enclosed, which includes the steps of adjusting the pH of said culture medium to be added to be higher than the pH which is optimum for culture of the cells, and/or adjusting the partial pressure of dissolved carbon dioxide of the culture medium to be added to be lower than the pressure which is optimum for culture of the cells, and/or adjusting the partial pressure of dissolved oxygen of the culture medium to be added to be higher than the pressure which is optimum for culture of the cells; and mixing the adjusted culture medium and the culture medium in said culture-container to adjust at least any of the pH, the partial pressure of dissolved carbon dioxide and the partial pressure of dissolved oxygen to be optimum for culture of said cells, thereby to culture the cells in the culture container.
1. A method of culturing cells in a culture container containing an original culture medium, comprising:
incubating the original culture medium and cells in the culture container to produce incubated culture medium and cultured cells;
mixing the incubated culture medium with a new culture medium in the culture container,
wherein
a pH of the new culture medium is higher than the pH of the original culture medium,
a partial pressure of dissolved carbon dioxide of the new culture medium is lower than the partial pressure of dissolved carbon dioxide of the original culture medium, and
a partial pressure of dissolved oxygen of the new culture medium is higher than the partial pressure of dissolved oxygen of the original culture medium, and wherein
the mixing raises the pH and the partial pressure of dissolved oxygen of the incubated culture medium and lowers the partial pressure of dissolved carbon dioxide of the incubated culture medium to optimum conditions for cell culture.
2. The method according to claim 1 , wherein the volume of the culture container is expanded to mix the new culture medium and the incubated culture medium in the culture container.
3. The method according to claim 2 , wherein the culture container is made of a soft packing material,
the culture container is divided into two or more chambers including a culture chamber and an extensible chamber by a predetermined member, and
the member and the culture container move relatively in accordance with an increase in number of cells in the culture chamber, whereby the volume of the culture chamber is increased.
4. The method according to claim 1 , wherein the pH of the incubated culture medium in the culture container is adjusted to 6.5 to 7.5 by the mixing.
5. The method according to claim 1 , wherein the partial pressure of dissolved carbon dioxide of the incubated culture medium in the culture container is adjusted to 20 mmHg to 50 mmHg by the mixing.
6. The method according to claim 1 , wherein the partial pressure of dissolved oxygen of the incubated culture medium in the culture container is adjusted to 115 mmHg to 170 mmHg by the mixing.
7. The method according to claim 1 , wherein a cell density in the culture container after the mixing is 5×10 3 /ml to 3×10 6 /ml.
8. The method according to claim 1 , the method further comprising culturing the cells after the mixing and collecting the cultured cells in a harvest container, wherein the harvest container is connected to the culture container by a tube.
9. The method according to claim 1 , wherein the new culture medium is transferred from a culture medium storage container, wherein the culture medium storage container is connected to the culture container by a tube.
10. The method according to claim 8 , wherein the new culture medium is transferred from a culture medium storage container, wherein the culture medium storage container is connected to the culture container by a tube.
11. The method according to claim 1 , wherein the culture container is a bag-shaped container made of a soft packing material.
12. A method of culturing cells in a cell culture system, the method comprising:
incubating the cells in a culture medium in a culture container,
transferring a new culture medium from a culture medium storage container to the culture container containing the culture medium and cells, wherein the culture medium storage container and the culture container are connected by a tube; and
mixing the culture medium with the new culture medium in the culture container, wherein
the pH of the new culture medium is higher than the pH of the culture medium in the culture container at the start of incubation,
the partial pressure of dissolved carbon dioxide of the new culture medium is lower than a partial pressure of dissolved carbon dioxide of the culture medium in the culture container at the start of incubation, and
the partial pressure of dissolved oxygen of the new culture medium is higher than a partial pressure of dissolved oxygen of the culture medium in the culture container at the start of incubation.
13. The method according to claim 12 , the method further comprising culturing the cells after the mixing and collecting the cultured cells in a harvest container, wherein the harvest container is connected to the culture container by a tube.
14. The method according to claim 13 , wherein the culture container is a bag-shaped container made of a soft packing material.
15. The method according to claim 13 , wherein the pH of the culture medium in the culture container is adjusted to 6.5 to 7.5 by the mixing.
16. The method according to claim 14 , wherein the partial pressure of dissolved carbon dioxide of the culture medium in the culture container is adjusted to 20 mmHg to 50 mmHg by the mixing.
17. The method according to claim 15 , wherein the partial pressure of dissolved oxygen of the culture medium in the culture container is adjusted to 115 mmHg to 170 mmHg by the mixing.
18. The method according to claim 9 , wherein the tube from the culture medium storage container passes through a culture medium adjustment apparatus.
19. The method according to claim 12 , wherein the tube from the culture medium storage container passes through a culture medium adjustment apparatus.