Photogenerated reagents
View Patent ↗This invention describes reagent precursors and methods for chemical and biochemical reactions. These reagent precursors that can be activated in solution upon irradiation to generate reagents required for the subsequent chemical reactions. Specifically, photogenerated reagents (PGR) are useful for controlling parallel combinatorial synthesis and various chemical and biochemical reactions.
1. A method for forming a biopolymer comprising:
(a) derivatizing a solid surface with a protected moiety containing at least one protective group
(b) contacting the solid surface with solution comprising at least one photogenerated reagent precursor and at least one co-reagent;
(c) irradiating the solution thereby generating a photogenerated reagent in at least a portion of the solution such that the protective group is removed from the protected moiety; and
(d) coupling a monomer to the deprotected moiety.
2. The method of claim 1 wherein the photogenerated reagent precursor is a photogenerated reagent acid precursor.
3. The method of claim 1 wherein the co-reagent is a base.
4. The method of claim 1 wherein the protected moiety is a protected nucleotide.
5. The method of claim 1 wherein the photogenerated reagent precursor is a photogenerated reagent base precursor.
6. The method of claim 1 wherein the co-reagent is an acid.
7. The method of claim 1 wherein the protected moiety is a protected amino acid.
8. The method of claim 1 wherein the co-reagent is a sensitizer or supersensitizer.
9. A method of deprotecting protected moieties attached to a solid surface and wherein the moieties have one or more protecting group(s) comprising a) contacting the protecting group moieties with a solution comprising one or more PGR-P(s) and one or more co-reagent(s) and b) irradiating the solution to produce one or more PGR(s) wherein the PGR(s) remove the protecting group(s) from the protected moiety.
10. The method of claim 9 wherein the photogenerated reagent precursor is a photogenerated reagent acid precursor.
11. The method of claim 9 wherein the co-reagent is a base.
12. The method of claim 9 wherein the protected moiety is a protected nucleotide.
13. The method of claim 9 wherein the photogenerated reagent precursor is a photogenerated reagent base precursor.
14. The method of claim 9 wherein the co-reagent is an acid.
15. The method of claim 9 wherein the protected moiety is a protected amino acid.
16. The method of claim 9 wherein the co-reagent is a sensitizer or supersensitizer.