Cell lines and methods for producing proteins
View Patent ↗The methods of the present invention involve the manipulation and/or propagation of oviduct tumor cells derived from either wild-type or transgenic avians.
1. A method comprising:
obtaining a cell from a tumor present in a tubular gland of a chicken oviduct; and culturing the obtained cell in cell culture medium.
2. The method of claim 1 wherein the tumor is an epithelial adenoma.
3. The method of claim 1 wherein the chicken is aged to at least about five years.
4. A method comprising,
i) obtaining at least one cell from tumorous tubular gland cells in an oviduct of a transgenic chicken, wherein the obtained at least one tumorous tubular gland cell of the transgenic chicken contains in the transgenic chicken genome a nucleotide sequence that is operably linked to a promoter and that encodes an exogenous protein;
ii) culturing the obtained at least one tumorous tubular gland in cell culture medium cell to express the exogenous protein in cell culture medium; and
iii) isolating the exogenous protein.
5. The method of claim 4 wherein the promoter is a heterologous promoter.
6. The method of claim 4 wherein the exogenous protein is selected from the group consisting of an antibody, a cytokine, a fusion protein, a growth factor, an enzyme, a structural protein, an interferon, a granulocyte-colony stimulating factor and an erythropoietin.
7. A method comprising,
i) obtaining at least one cell from tumorous tubular gland cells in a chicken oviduct;
ii) culturing the obtained at least one cell in cell culture medium;
iii) introducing into the cultured cell(s) and progeny therein a nucleotide sequence operably linked to a promoter, wherein the nucleotide sequence comprises a coding sequence encoding a heterologous protein wherein the nucleotide sequence is expressed to produce the heterologous protein; and
iv) isolating the heterologous protein.
8. The method of claim 7 wherein the promoter is selected from the group consisting of a cytomegalovirus (CMV) promoter, and a rous-sarcoma virus (RSV) promoter.
9. The method of claim 7 wherein the heterologous protein is a therapeutic protein.
10. The method of claim 7 , wherein the nucleotide sequence further comprises a sequence encoding a selectable marker operably linked to an expression control sequence and wherein the method further comprises selecting for tumor cells that express the marker.
11. The method of claim 7 wherein the chicken is at least about five years of age.
12. The method of claim 7 wherein the tumorous tubular gland cells are epithelial adenoma.
13. The method of claim 7 wherein the heterologous protein is selected from the group consisting of an antibody, a cytokine, a fusion protein, a growth factor, an enzyme, a structural protein, an interferon, a granulocyte-colony stimulating factor, and an erythropoietin.