Engineered cleavage half-domains
View Patent ↗Disclosed herein are engineered cleavage half-domains; fusion polypeptides comprising these engineered cleavage half-domains; polynucleotides encoding the engineered cleavage half-domains and fusion proteins; and cells comprising said polynucleotides and/or fusion proteins. Also described are methods of using these polypeptides and polynucleotides, for example for targeted cleavage of a genomic sequence.
1. A polypeptide comprising an engineered FokI cleavage half-domain, wherein
(i) the engineered cleavage half-domain comprises a mutation in one or more wild-type amino acid residue 483, 486, 487, 490, 499, 538 or combinations thereof, wherein the amino acid residues are numbered relative to full length wild-type FokI;
(ii) the engineered cleavage half-domain forms an obligate heterodimer with a wild-type cleavage half-domain or a second engineered cleavage half-domain; and
(iii) if the engineered cleavage half-domain comprises a mutation at amino acid residue 490, it further comprises at least one additional amino acid mutation.
2. The polypeptide of claim 1 , wherein the amino acid residues at positions 490 and 538 are mutated.
3. The polypeptide of the claim 2 , wherein the engineered cleavage half-domain comprises the polypeptide designated E490K:1538K.
4. The polypeptide of claim 1 , wherein the wild-type amino acid at position 486 is mutated.
5. The polypeptide of claim 4 , wherein the engineered cleavage half-domain comprises the polypeptide designated Q486E.
6. The polypeptide of claim 1 , wherein the wild-type amino acid residue at position 499 is mutated.
7. The polypeptide of claim 6 , wherein the engineered cleavage half-domain comprises the polypeptide designated 1499L.
8. The polypeptide of claim 7 , wherein the engineered cleavage half-domain comprises the polypeptide designated Q486E:1499L.
9. A heterodimer comprising an engineered cleavage half-domain of claim 1 and a wild-type Fokl cleavage half-domain.
10. The heterodimer of claim 9 , wherein the engineered cleavage half-domain comprises the polypeptide designated E490K:1538K.
11. A heterodimer comprising a first engineered cleavage half-domain of claim 1 and a second engineered cleavage half-domain.
12. The heterodimer of claim 11 , wherein the first engineered cleavage half-domain comprises mutation in amino acid residue 490 and/or amino acid residue 538.
13. The heterodimer of claim 11 , wherein the second engineered cleavage half-domain comprises a mutation in amino acid residue 486 and/or amino acid residue 499.
14. The polypeptide of claim 1 , further comprising a zinc finger protein DNA-binding domain.
15. An isolated cell comprising the polypeptide of claim 1 .