Layered flask cell culture system
View Patent ↗The present invention is a cell cultivating vessel or device, such as a single or multitier flask, including a cover having a top plate, a side wall and a resealable port; an intermediate tray for receiving cells and cell culture media, having a bottom plate, a side wall, and a gap region formed between an interior upwardly angled lip located on an interior portion of the intermediate tray bottom plate and an adjacent outwardly angled side wall portion of the intermediate tray bottom plate, wherein the lip has a outwardly swooping curvilinear edge feature; and a base tray for receiving the cells and cell culture media, including a bottom plate and a side wall. The intermediate tray is positioned between the cover and the base tray, such that the gap region of the intermediate tray bottom plate is in alignment with the port located on the cover, resulting in the port, the intermediate tray and the base tray in fluid communication with one another which provides direct access, such as by a user to remove and/or add cells, cell media, and nutrients located on each of the intermediate and/or the base trays. Alternatively, the cell cultivating flask includes a plurality of intermediate trays stacked on top of one another and the gap regions of each intermediate tray are in alignment with each other and with the port on the cover.
1. A cell cultivating flask comprising:
a) a cover having a substantially horizontal surface and a resealable port;
b) two or more identical intermediate trays each comprising a substantially flat portion, an upwardly angled lip, and a gap region, wherein the gap region is formed between the edge of the lip and a sidewall of the flask, and wherein at least a portion of the gap region aligns with the port; and
c) a base tray comprising a first and second substantially flat portions, the second substantially flat portion located higher than the first substantially flat portion, and an interconnecting portion which is at an angle to interconnect the first and second portions, where at least a portion of the second substantially flat portion aligns with the port and the gap region.
2. The flask of claim 1 , wherein the angle of the interconnecting portion in the base tray is from about 10 degrees to about 60 degrees.
3. The flask of claim 2 , wherein the angle is from about 10 degrees to about 45 degrees.
4. The flask of claim 2 , wherein the angle is from about 15 degrees to about 30 degrees.
5. The flask of claim 2 , wherein the angle is about 22 degrees.
6. The flask of claim 1 , wherein the resealable port further comprises a threaded cap.
7. The flask of claim 6 , wherein the threaded cap comprises a gas permeable hydrophobic membrane or filter.
8. The flask of claim 7 , wherein the gas permeable hydrophobic membrane or filter has a pore size less than about 0.65 microns.
9. The flask of claim 7 , wherein the gas permeable hydrophobic membrane or filter has a pore size less than about 0.22 microns.
10. The flask of claim 1 , wherein the flask comprises two intermediate trays.
11. The flask of claim 1 , wherein the flask comprises at least four intermediate trays.
12. The flask of claim 1 , wherein the flask comprises at least eight intermediate trays.
13. The flask of claim 1 , further comprising a camera mounted to the cover or base tray.
14. The flask of claim 1 , further comprising an overmolded plastic seal around the perimeter of the flask.
15. The flask of claim 1 , further comprising a non-skid button on the base.
16. The flask of claim 1 , further comprising non-slip gripping ribs.
17. The flask of claim 1 , further comprising a lens in the cover or base tray.