Array for rapid detection of a microorganism
View Patent ↗A method for detecting a microorganism or class of microorganisms is provided. More specifically, the method employs an array that contains a plurality of discrete regions (referred to as “addresses”) spaced apart on a solid support in a predetermined pattern. The addresses are selected so that the array provides a distinct spectral response (e.g., pattern of colors) or “fingerprint” for a particular microorganism or class of microorganisms. For example, the array may provide a certain spectral response in the presence of one microorganism or class of microoryanisms (e.g., gram-negative bacteria), but provide a completely different spectral response in the presence of another microorganism or class of microorganisms (e.g., gram-positive bacteria). Detection of the spectral response provided by the array may thus allow for differentiation between microorganisms.
1. An array for detecting a microorganism in a sample, the array comprising a plurality of individual array addresses spaced apart in a predetermined pattern on a solid support, wherein at least one of the addresses contains a solvatochromatic colorant in an amount sufficient to undergo a detectable color change in the presence of a broad spectrum of microorganisms, wherein the broad spectrum of microorganisms includes S. aureus, E. coli, C. albicans , or combinations thereof, and wherein at least a portion of the addresses contain a differentiating colorant so that the array produces a visually observable spectral response that is distinct for one or more microorganisms, wherein the solvatochromatic colorant is an N-phenolate betaine and the differentiating colorant is a pH sensitive colorant or a metal complexing colorant.
2. The array of claim 1 , wherein the pH sensitive colorant is a phthalein, hydroxyanthraquinone, arylmethane, aromatic azo, or a derivative thereof.
3. The array of claim 1 , wherein the array contains from 2 to 50 individual array addresses.
4. The array of claim 1 , wherein the array contains from 3 to 40 individual array addresses.
5. The array of claim 1 , wherein at least two of the addresses are spaced apart a distance of from about 0.01 to about 100 millimeters.
6. The array of claim 1 , wherein at least two of the addresses are spaced apart a distance of from about 0.1 to about 50 millimeters.
7. The array of claim 1 , wherein the spectral response is distinct for one or more microorganisms at a concentration of about 1×10 3 or more colony forming units per milliliter of the sample.
8. The array of claim 1 , wherein the spectral response is distinct for one or more microorganisms at a concentration of about 1×10 6 or more colony forming units per milliliter of the sample.
9. The array of claim 1 , wherein the N-phenolate betaine is Reichardt's dye.