Predictors of patient response to treatment with EGF receptor inhibitors
View Patent ↗The present invention provides methods and compositions to facilitate determining whether an EGFR-expressing cancer in an individual is an EGFR inhibitor-responsive cancer, as well as methods for determining the likelihood that a patient having an EGFR-expressing cancer will exhibit a beneficial response to an EGFR inhibitor therapy. The methods generally involve determining a normalized expression level of a gene product that correlates with EGFR inhibitor responsiveness.
1. A method for predicting a likelihood that a human patient with an epidermal growth factor receptor (EGFR)—expressing colorectal cancer will exhibit a beneficial response to an EGFR inhibitor comprising:
(a) measuring, in a tumor sample obtained from the patient, a level of an RNA transcript, or its expression product, for each of solute carrier family 26, member 3 (SLC26A3), amphiregulin (AREG), epiregulin (EREG) and dual specificity phosphatase 6 (DUSP6),
(b) normalizing the level of the RNA transcript, or its expression product, for each of SLC26A3, AREG, EREG, and DUSP6 to obtain a normalized expression level for each of SLC26A3, AREG, EREG, and DUSP6; and
(c) using the normalized expression level to determine the likelihood that the patient will exhibit a beneficial response to an EGFR inhibitor,
wherein the normalized expression levels of SLC26A3, AREG, and EREG are positively correlated with the likelihood that the patient will exhibit a beneficial response to the EGFR inhibitor, and wherein the normalized expression level of DUSP6 is negatively correlated with the likelihood that the patient will exhibit a beneficial response to the EGFR inhibitor.
2. The method of claim 1 , wherein the tumor sample is obtained from a tissue biopsy.
3. The method of claim 1 , wherein the EGFR inhibitor is an antibody specific for EGFR.
4. The method of claim 3 , wherein the EGFR inhibitor is cetuximab.
5. The method of claim 1 , wherein the EGFR inhibitor is a small molecule.
6. The method of claim 5 , wherein the small molecule is an EGFR-selective tyrosine kinase inhibitor.
7. The method of claim 1 , wherein the normalized expression level is determined relative to an expression level of at least one reference gene.
8. The method of claim 1 , wherein the levels of the SLC26A3, AREG, EREG, and DUSP6 RNA transcripts are measured.
9. The method of claim 8 , wherein the levels of the SLC26A3, AREG, EREG, and DUSP6 RNA transcripts are measured by reverse transcriptase polymerase chain reaction (RT-PCR).
10. The method of claim 1 , wherein beneficial response is expressed in terms of Overall Response Rate (ORR) or Disease Control (DC).
11. The method of claim 1 , wherein the normalized expression level is weighted by its contribution to response to the EGFR inhibitor.
12. The method of claim 1 , further comprising the step of creating a report summarizing said prediction.