Method of Analyzing a BRCA2 Gene in a Human Subject
Five novel DNA and protein sequences have been determined for the BRCA2 gene, as have been ten polymorphic sites and their rates of occurrence in the normal alleles of BRCA2. The sequences BRCA2 (omi 1-5) and the ten polymorphic sites will provide greater accuracy and reliability for genetic testing. One skilled in the art will be better able to avoid misinterpretations of changes in the gene and/or protein sequence, determine the presence of a normal sequence, and of mutations of BRCA2. This invention is also related to a method of performing gene therapy with BRCA2 (omi 1-5) coding sequences or fragments thereof. This invention is further related to protein therapy with BRCA2 (omi 1-5) proteins or their functional equivalents.
1 - 60 . (canceled)
61 . An isolated nucleic acid comprising:
(a) 18 contiguous nucleotides spanning the 3′ exon/intron junction of exon 15 according to FIG. 2D ;
(b) 18 contiguous nucleotides spanning the 5′ exon/intron junction of exon 16 according to FIG. 2D ;
(c) the nucleotide
sequence 5′-GTGTTCTCATAAACAGgtatgtgt-3′;
(d) the nucleotide
sequence 5′-tttttcttttttgtgtgtgtttattttgtgtagCTGTA
TACGTATGGCGTTTC-3′;
(e) the nucleotide sequence of exon 15 and having the nucleotide
sequence 5′- GTGTTCTCATAAACAGgtatgtgt-3′;
(f) the nucleotide sequence of SEQ ID NO:2; or
(g) the nucleotide sequence of SEQ ID NO:3.
62 . A method of amplifying exon 15 of a BRCA2 gene, wherein the sequence of the last 16 nucleotides of exon 15 is 5′-GTGTTCTCATAAACAG-3′, the method comprising:
obtaining a sample containing genomic DNA from a human subject;
amplifying exon 15 or two or more portions thereof from said genomic DNA.
63 . The method of claim 62 , wherein said amplifying step comprises PCR to obtain (1) a first isolated nucleic acid comprising the 5′ intron/exon junction of exon 15 according to SEQ ID NO:2 and (2) a second isolated nucleic acid comprising the 3′ exon/intron junction of exon 15 according to SEQ ID NO:2.
64 . A method of amplifying exon 16 of a BRCA2 gene, wherein the sequence of the first 20 nucleotides of exon 16 is 5′-CTGTATACGTATGGCGTTTC-3′, the method comprising:
obtaining a sample containing genomic DNA from a human subject;
amplifying exon 16 or two or more portions thereof from said genomic DNA.
65 . The method of claim 64 , wherein said amplifying step comprises PCR to obtain (1) a first isolated nucleic acid comprising the 5′ intron/exon junction of exon 16 according to SEQ ID NO:3 and (2) a second isolated nucleic acid comprising the 3′ exon/intron junction of exon 16 according to SEQ ID NO:3.