Methods for identifying and monitoring drug side effects
The present invention relates generally to methods for identifying drug side effects by detecting perturbations in organ-specific molecular blood fingerprints. The invention further relates to methods for identifying drug-specific organ-specific molecular blood fingerprints. As such, the present invention provides compositions comprising organ-specific proteins, detection reagents for detecting such proteins, and panels and arrays for determining organ-specific molecular blood fingerprints.
1. A method for detecting a drug side effect comprising measuring in the blood of a subject taking the drug the level of a plurality of organ-specific proteins secreted from an organ wherein the levels of the plurality of organ-specific proteins together provide an organ-specific molecular blood fingerprint that indicates a drug side effect on the organ in the subject.
2. The method of claim 1 wherein the level of the plurality of organ-specific proteins is measured using a method selected from the group consisting of mass spectrometry, immunoassay, tandem mass spectrometry, ELISA, Western blot, microfluidics/nanotechnology sensors, and aptamer capture assay.
3. The method of claim 2 wherein the level of the plurality of organ-specific proteins is measured using tandem mass spectrometry.
4. The method of claim 2 wherein the level of the plurality of organ-specific proteins is measured using ELISA.
5. The method of claim 1 wherein the plurality of organ-specific proteins comprises 5 organ-specific proteins.
6. The method of claim 1 wherein the plurality of organ-specific proteins comprises 20 organ-specific proteins.
7. The method of claim 1 wherein the organ-specific proteins comprise liver-specific proteins.
8. The method of claim 1 wherein the organ-specific proteins comprise kidney-specific proteins.
9. The method of claim 1 wherein the organ-specific proteins comprise prostate-specific proteins.
10. The method of claim 2 wherein the level of each of the plurality of organ-specific proteins is measured using an antibody array.
11. The method of claim 1 wherein the plurality of organ-specific proteins comprises 10 organ-specific proteins.
12. The method of claim 1 wherein the plurality of organ-specific proteins comprises 50 organ-specific proteins.
13. The method of claim 1 wherein the organ-specific proteins are from an organ other than the expected therapeutic target of the drug.
14. The method of claim 1 wherein measuring the level of the plurality of organ-specific proteins is accomplished by detecting the level of each of the plurality of organ-specific proteins in a blood sample from the subject.
15. The method of claim 1 wherein a statistically significant altered level of one or more of the plurality of organ-specific proteins in the blood is indicative of the presence or absence of a drug side effect.
16. The method of claim 15 where the altered level of one or more of the plurality of organ-specific proteins is determined by comparing the level of each of the plurality of organ-specific proteins in the blood sample from the subject to a level of each of the plurality of organ-specific proteins in a control sample of blood.
17. The method of claim 1 wherein the plurality of organ-specific proteins are secreted from the same organ.
18. The method of claim 1 wherein the plurality of organ-specific proteins comprises 100 organ-specific proteins.
19. The method of claim 1 wherein the plurality of organ-specific proteins comprises 5 organ-specific proteins from each particular organ.
20. The method of claim 1 wherein the plurality of organ-specific proteins comprises 10 organ-specific proteins from each particular organ.
21. The method of claim 1 wherein the plurality of organ-specific proteins comprises 20 organ-specific proteins from each particular organ.
22. The method of claim 1 wherein the plurality of organ-specific proteins comprises 50 organ-specific proteins from each particular organ.