Compositions and methods of selective nucleic acid isolation
The invention relates to methods for isolating and/or identifying nucleic acids. The invention also provides kits for isolating and/or identifying nucleic acids.
1. A kit comprising:
a solid phase;
a nucleic acid binding buffer, wherein both DNA and RNA bind the solid phase under conditions generated by the nucleic acid binding buffer; and
a selective DNA binding buffer, wherein the conditions generated by the selective DNA binding buffer allow selective binding of DNA to the solid phase, wherein the pH of the DNA binding buffer is equal to or above 8.0.
2. The kit of claim 1 , further comprising an RNA binding buffer, wherein the conditions generated by the RNA binding buffer allow RNA to bind to a solid phase, wherein the RNA binding buffer is a neutral or acidic pH.
3. The kit of claim 1 , wherein the solid phase is a siliceous material.
4. The kit of claim 3 , wherein the siliceous material is selected from at least one of silica, silica dioxide, diatomaceous earth, glass, Celite, and silica gel.
5. The kit of claim 3 , wherein the solid phase is in a form selected from at least one of a particle, a bead, a membrane, a frit, a surface within a chamber, and a side of a container.
6. The kit of claim 1 , wherein the selective DNA binding buffer comprises a large anion, wherein the large anion is at least as large as a bromide ion.
7. The kit of claim 6 , wherein the large anion is selected from at least one of picrate, tannate, tungstate, molybdate, perchlorate, and sulfosalicylate.
8. The kit of claim 6 , wherein the large anion is selected from at least one of trichloroacetate, tribromoacetate, thiocyanate, and nitrate.
9. The kit of claim 6 , wherein the large anion is selected from at least one of iodide and bromide.
10. The kit of claim 1 , wherein the nucleic acid binding buffer has a pH of 6 to less than pH 7.
11. The kit of claim 1 , wherein the nucleic acid binding buffer has a pH of 6.
12. The kit of claim 1 , wherein the pH of the DNA binding buffer is between 8.0 and 12.0.
13. The kit of claim 12 , wherein the pH of the DNA binding buffer is equal to, or above 9.0.
14. The kit of claim 13 , wherein the pH of the DNA binding buffer is equal to, or above 10.0.
15. The kit of claim 1 , wherein the solid phase is a siliceous material, wherein the nucleic acid binding buffer has a pH of 6 to less than pH 7, and wherein the pH of the DNA binding buffer is between 8.0 and 12.0.