Method of producing amino acid metal chelates
View Patent ↗A method of producing amino acid metal chelates includes producing an amino acid ligand by enzymatically hydrolyzing bacterial cells, and reacting the amino acid ligand with a metal cation.
1. A method of producing amino acid metal chelates, comprising:
producing an amino acid ligand by enzymatically hydrolyzing bacterial cells; and
reacting the amino acid ligand with a metal cation;
wherein:
the bacterial cells are obtained as a by-product of amino acid production; and
the bacterial cells are provided in spent media from production of at least one amino acid selected from the group consisting of arginine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan, valine, glycine, serine, cysteine, tyrosine, alanine, aspartic acid, glutamic acid, proline, asparagine, and glutamine.
2. The method of claim 1 , wherein producing amino acid metal chelates comprises producing metal chelates of at least one of free amino acids, dipeptides and tripeptides.
3. The method of claim 1 , wherein enzymatically hydrolyzing bacterial cells comprises hydrolyzing a cell cream having a solids content of from about 1 to about 30 weight percent.
4. The method of claim 1 , wherein enzymatically hydrolyzing bacterial cells comprises hydrolyzing a cell cream having a nitrogen content of from about 0.12 to about 4 weight percent.
5. The method of claim 1 , wherein enzymatically hydrolyzing bacterial cells comprises hydrolyzing with at least one enzyme that is capable of hydrolyzing bacterial proteins into at least one of free amino acids and short peptides.
6. The method of claim 1 , wherein enzymatically hydrolyzing bacterial cells comprises hydrolyzing with a mixture of one or more enzymes of plant origin, enzymes of animal origin, enzymes of bacterial origin and enzymes of fungal origin.
7. The method of claim 1 , wherein enzymatically hydrolyzing bacterial cells comprises hydrolyzing with at least one member selected from the group consisting of proteases, lipases and amylases.
8. The method of claim 1 , wherein enzymatically hydrolyzing bacterial cells comprises hydrolyzing with at least one member selected from the group consisting of pancreatin, papain, bromelain, bacterial protease, fungal protease, trypsin, chymotrypsin, pepsin and rennin.
9. The method of claim 1 , wherein producing an amino acid ligand by enzymatically hydrolyzing bacterial cells comprises combining an enzyme and the bacterial cells in a weight ratio of from about 0.1 to about 10 g of enzyme per 100 g of nitrogen content of bacterial cells.
10. The method of claim 1 , wherein producing an amino acid ligand by enzymatically hydrolyzing bacterial cells comprises reacting an enzyme and the bacterial cells in the presence of a catalyst.
11. The method of claim 10 , wherein the catalyst comprises at least one member selected from the group consisting of iron, copper, cobalt, nickel, boron, magnesium, calcium and rare earth metals.
12. The method of claim 1 , wherein reacting the amino acid ligand with a metal cation comprises reacting the amino acid ligand with a metal cation having a valence of two or more.
13. The method of claim 1 , wherein reacting the amino acid ligand with a metal cation comprises reacting the amino acid ligand with at least one metal cation selected from the group consisting of calcium, magnesium, chromium, manganese, iron, copper, cobalt, boron, molybdenum, vanadium and zinc.
14. The method of claim 1 , wherein reacting the amino acid ligand with a metal cation comprises reacting the amino acid ligand with a salt of the metal cation.
15. A method of producing amino acid metal chelates, comprising:
producing an amino acid ligand by enzymatically hydrolyzing bacterial cells; and
reacting the amino acid ligand with a metal cation;
wherein enzymatically hydrolyzing bacterial cells comprises hydrolyzing with at least one enzyme that is capable of hydrolyzing bacterial proteins into at least one of free amino acids and short peptides.
16. The method of claim 15 , wherein producing amino acid metal chelates comprises producing metal chelates of at least one of free amino acids, dipeptides and tripeptides.
17. The method of claim 15 , wherein the bacterial cells are obtained as a by-product of an industrial process that generates bacterial mass.
18. The method of claim 17 , wherein the industrial process is selected from the group consisting of ethanol production, organic acid production, and waste-water treatment.
19. The method of claim 15 , wherein the bacterial cells are obtained as a by-product of amino acid production.
20. The method of claim 19 , wherein the bacterial cells are provided in spent media from production of at least one amino acid selected from the group consisting of lysine, threonine, tryptophan and glutamic acid.
21. The method of claim 15 , wherein enzymatically hydrolyzing bacterial cells comprises hydrolyzing a cell cream having a solids content of from about 1 to about 30 weight percent.
22. The method of claim 15 , wherein enzymatically hydrolyzing bacterial cells comprises hydrolyzing a cell cream having a nitrogen content of from about 0.12 to about 4 weight percent.
23. The method of claim 15 , wherein enzymatically hydrolyzing bacterial cells comprises hydrolyzing with a mixture of one or more enzymes of plant origin, enzymes of animal origin, enzymes of bacterial origin and enzymes of fungal origin.
24. The method of claim 15 , wherein enzymatically hydrolyzing bacterial cells comprises hydrolyzing with at least one member selected from the group consisting of proteases, lipases and amylases.
25. The method of claim 15 , wherein enzymatically hydrolyzing bacterial cells comprises hydrolyzing with at least one member selected from the group consisting of pancreatin, papain, bromelain, bacterial protease, fungal protease, trypsin, chymotrypsin, pepsin and rennin.
26. The method of claim 15 , wherein producing an amino acid ligand by enzymatically hydrolyzing bacterial cells comprises combining an enzyme and the bacterial cells in a weight ratio of from about 0.1 to about 10 g of enzyme per 100 g of nitrogen content of bacterial cells.
27. The method of claim 15 , wherein producing an amino acid ligand by enzymatically hydrolyzing bacterial cells comprises reacting an enzyme and the bacterial cells in the presence of a catalyst.
28. The method of claim 27 , wherein the catalyst comprises at least one member selected from the group consisting of iron, copper, cobalt, nickel, boron, magnesium, calcium and rare earth metals.
29. The method of claim 15 , wherein reacting the amino acid ligand with a metal cation comprises reacting the amino acid ligand with a metal cation having a valence of two or more.
30. The method of claim 15 , wherein reacting the amino acid ligand with a metal cation comprises reacting the amino acid ligand with at least one metal cation selected from the group consisting of calcium, magnesium, chromium, manganese, iron, copper, cobalt, boron, molybdenum, vanadium and zinc.
31. The method of claim 15 , wherein reacting the amino acid ligand with a metal cation comprises reacting the amino acid ligand with a salt of the metal cation.