Liquid separation from adipose tissue

1. A method of removing a liquid phase from a whole adipose tissue sample, comprising:

moving a collected whole adipose tissue sample into an adipose particle region defined by a central volume of a separation container;

spinning the separation container to move at least a portion of the whole adipose tissue sample adjacent to a passage portion of the separation container;

forcing at least a portion of a liquid phase of the whole adipose tissue to move through the passage portion while maintaining the adipose particles within the adipose particle region of the separation container;

collecting the portion of the liquid phase forced through the passage portion in a liquid collection region of the separation container wherein the liquid phase includes water; and

withdrawing the adipose particles from the adipose particle region through a withdrawal tube;

wherein spinning the separation container includes spinning the separation container around a central axis of the separation container to form a centrifugal force towards an outer wall of the separation container;

wherein the central volume is coaxial with the central axis and forcing at least a portion of the liquid phase includes using the centrifugal force to force the at least a portion of the liquid phase through the passage portion.

2. The method of claim 1 , wherein withdrawing the adipose particles includes withdrawing adipose particles substantially devoid of the liquid phase.

3. The method of claim 2 , wherein the adipose particles substantially devoid of the liquid phase includes about 50 percent less liquid than the whole adipose tissue sample.

4. The method of claim 2 , wherein the adipose particles substantially devoid of the liquid phase are positioned within a patient and are appropriate for maintaining a selected contour of the patient with the adipose particles substantially devoid of the liquid phase.

5. The method of claim 1 , wherein the central axis of the separation container is defined within the separation container and the centrifugal force is away from the central axis to force the liquid phase to separate from other phases of the collected whole adipose tissue sample to move the liquid phase through the passage portion.

6. The method of claim 5 , further comprising:

selecting a passage diameter of the passage portion to have a diameter to allow substantially only a water or aqueous material to move through the passage portion while maintaining the adipose particles that include a fat portion of the whole adipose tissue sample within the adipose particle region.

7. The method of claim 6 , wherein whole adipose tissue sample and the adipose particles are maintained within the same adipose particle region to allow for withdrawal of the adipose particles from the adipose particle region after the liquid phase has been forced through the passage portion;

wherein withdrawing the adipose particles from the adipose particle region occurs through a withdrawal port while maintaining the separation container substantially closed.

8. A method of removing a liquid phase from a whole adipose tissue sample, comprising:

placing a whole adipose tissue sample into a first separation region of a separation container defined by a wall surrounding a central axis of the first separation region, wherein the whole adipose tissue sample includes adipose particles and a liquid phase;

spinning the separation container about the central axis of the first separation region to place a force on the obtained whole adipose tissue sample placed in the separation container against the wall to cause a volume of the liquid phase of the whole adipose tissue to be removed through a passage portion defined through the wall to a second separation region of the separation container while spinning the separation container and while at least a portion of the adipose particles of the whole adipose tissue sample are contained within the first separation region, wherein the second separation region surrounds at least a portion of the first separation region;

collecting at least the portion of the adipose particles in the first separation region after the volume of the liquid phase has been removed through the passage portion and is contained in the second separation region of the separation container; and

withdrawing from the first separation region at least a portion of the at least the portion of the adipose particles that includes a fat portion of the whole adipose tissue sample.

9. The method of claim 8 , further comprising:

placing the separation container within an exterior container;

wherein withdrawing at least the portion of at least the portion of the adipose particles from the first separation region is performed through the exterior container.

10. The method of claim 9 , further comprising:

connecting the separation container to a motor while placed within the exterior container; and

operating the motor to spin the separation container to cause spinning the separation container;

wherein the motor is operated to spin the separation container at least about 2000 rotations per minute for at least about 30 seconds.

11. The method of claim 10 , further comprising:

providing the passage portion to have a passage diameter appropriate to contain the adipose particles within the first separation region.

12. The method of claim 11 , wherein the spinning the separation container forces the whole adipose tissue sample against the passage portion such that the volume of the liquid phase is removed through the passage portion and further operable to move through a portion of the whole adipose tissue sample.

13. The method of claim 9 , wherein collecting adipose particles further includes:

ceasing spinning the separation container; and

allowing at least the portion of the adipose particles to move to a sump defined within the first separation region of the separation container separate from the second separation region.

14. The method of claim 13 , wherein withdrawing the adipose particles includes withdrawing the adipose particles from the sump within the separation container through an access port in both the exterior container and the separation container.

15. The method of claim 8 , further comprising delivering the adipose particles to a surface.

16. The method of claim 8 , further comprising:

providing a filter between the first separation region and the second separation region, wherein the volume of the liquid phase passes through the filter as the volume of the liquid phase is removed to the second separation region.

17. The method of claim 16 , wherein the filter includes a felt.

18. A method of removing a liquid phase from a whole adipose tissue sample, comprising:

positioning a whole adipose tissue sample in an adipose particle collection region, wherein the adipose particle collection region includes at least a portion of a central volume of a separation container;

spinning the separation container to apply a force to the whole adipose tissue sample to force a selected volume of a liquid phase of the whole adipose tissue sample through a passage portion and a filter between the adipose particle collection region and a liquid collection region, wherein at least one of the passage portion or the filter allows substantially only the liquid phase to pass through the filter as the volume of the liquid phase is removed to the liquid collection region and sequestering the selected volume of the liquid phase in the liquid collection region;

collecting the remaining adipose tissue sample in the adipose particle collection region; and

withdrawing the collected remaining adipose tissue sample from the adipose particle collection region;

wherein spinning the separation container includes operating a motor to apply a force to spin the separation container to cause the separation container to spin around a central axis of the separation container, wherein at least the portion of the central volume is coaxial with the central axis.

19. The method of claim 18 , wherein collecting the remaining adipose tissue sample includes operating the motor to cease the spinning of the separation container to remove the force after the selected volume of the liquid phase is sequestered and allowing the remaining adipose tissue sample to move in the adipose particle region.

20. The method of claim 19 , further comprising:

closing the separation container;

wherein positioning the whole adipose tissue sample in the adipose particle collection region of the separation container occurs after closing the separation container.

21. The method of claim 20 , further comprising:

enclosing the separation container within an exterior container;

wherein withdrawing the collected remaining adipose tissue sample from the adipose particle collection region occurs while the separation container is enclosed in the exterior container.

22. The method of claim 19 , wherein collecting the remaining adipose tissue sample includes collecting a tissue sample having a selected portion of a liquid removed from the whole adipose tissue sample such that the remaining adipose tissue sample includes a higher concentration of adipose particles include adipocytes than the whole adipose tissue sample positioned in the adipose particle collection region prior to collecting the remaining adipose tissue sample from within the adipose particle collection region.

23. The method of claim 22 , further comprising:

interconnecting a whole adipose tissue sample holding container with the separation container;

interconnecting a remaining adipose tissue sample container with the separation container; and

maintaining a sterility of the separation container and the adipose tissue sample and the remaining adipose tissue sample;

wherein positioning a whole adipose tissue sample is performed after interconnecting the whole adipose tissue sample holding container with the separation container and withdrawing the remaining adipose tissue sample occurs after connecting the adipose tissue sample container;

wherein the adipose tissue sample and the remaining adipose tissue sample are maintained in the same region of the separation container.

24. The method of claim 19 , wherein the force is applied away from the central axis of the separation container towards a first outer wall of the separation container;

wherein a portion of the first outer wall defines the passage portion and a second outer wall defines at least a portion of a holding area to hold the sequestered liquid phase.

25. The method of claim 8 , wherein the fat portion includes adipocytes and the liquid phase includes at least liquid or proteins between adipocytes in the whole adipose tissue sample, wherein removal of the volume of the liquid phase from the adipose particles results in firmer and denser adipose particles than the whole adipose tissue sample.