[1, 2, 4] Triazolo [1, 5-A] pyrimidine derivatives as chromatographic adsorbent for the selective adsorption of IGG

1. A method for affinity chromatography, comprising the step of selectively adsorbing IgG, fragments thereof and/or complexes involving IgG or fragments thereof on a chromatographic adsorbent, using a compound comprising the following formula

and its corresponding enol-form,

wherein

X represents O, S, or NH;

R 1 represents H, C 1-6 alkyl, C 1-6 alkoxy, C 1-6 alkoxy-C 1-6 alkyl, Ar, —C(O)NHR 3 , —C(O)—R 3 or halo;

R 2 represents H, C 1-3 alkyl or halo;

R 3 represents H, C 1-6 alkyl, C 1-6 alkoxy, C 1-6 alkoxy-C 1-6 alkyl or Ar;

n represents 0, 1, 2 or 3;

Y represents a carrier;

Ar represents a C 6-10 aryl group, which group is optionally substituted by one or more substituents selected from —OH, cyano, halo, nitro, C 1-6 alkyl and alkoxy.

2. The method of claim 1 , wherein the carrier Y is selected from cellulose, starch, dextran, agar, agarose, polyacrylamide, poly(meth)acrylate, a polyvinyl hydrophilic polymer, polystyrene and polysulfone, silica, alumina, titania oxide, zirconia oxide, a polysaccharide-synthetic polymer, a polysaccharide-mineral structure, or a synthetic polymer-mineral structure.

3. The method of claim 1 , wherein R 1 represents H, n represents 0, X represents NH and Y represents agarose.

4. The method of claim 3 , further comprising a spacer molecule between X and Y.

5. The method of claim 4 , wherein Y represents HP-Sepharose, and hexamethylene diamine is the spacer molecule.

6. The method of claim 1 , further comprising a spacer molecule between X and Y.

7. The method of claim 6 , wherein R 1 represents H, n represents 0, X represents NH, Y represents HP-Sepharose, and hexamethylene diamine is the spacer molecule.

8. The method of claim 1 , wherein the carrier material Y is in the form of beads, irregularly shaped particles, fibers, membranes, flat structure or porous mineral materials.